تاثیر منابع مختلف سلنیوم بر فراسنجه‌های کیفی اسپرم خروس های مادر گوشتی مسن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران

4 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی گلستان، گرگان، ایران

چکیده

یکی از روش های بهبود در باروری، مکمل‌سازی عناصری نظیر سلنیوم به جیره است. اثرات مکمل‌سازی سلنیوم متفاوت است و شدیداً تحت تأثیر منبع و مقدار سلنیوم جیره قرار می گیرد لذا این پژوهش به منظور تاثیر منابع مختلف سلنیوم بر فراسنجه‌های کیفی اسپرم خروس های مادر گوشتی مسن انجام شد. این پژوهش با استفاده از 120 قطعه خروس مادر گوشتی راس 308 با سن 45 هفته در قالب طرح کاملاً تصادفی با چند نمونه در هر واحد آزمایشی 10 تیمار با 4 تکرار و 3 پرنده در داخل هر تکرار اجرا شد. سه منبع مختلف سلنیوم شامل سلنیت سدیم، سلنیوم آلی و نانو بایوکیلات سلنیوم، هر کدام در سه سطح صفر، 0/15، 0/30 و 0/45 میلی‌گرم در کیلوگرم به جیره افزوده شدند. بعد از دو هفته تغذیه با جیره پایه، خروس‌ها به مدت 40 روز و از ابتدای سن 47 هفتگی تیمارهای آزمایشی را دریافت کردند. هر 10 روز یکبار اسپرم‌گیری به روش مالش شکمی از خروس‌ها صورت گرفت سپس فراسنجه‌های کیفی اسپرم خروس ها شامل حجم منی، غلظت، درصد جنبایی، درصد زنده‌مانی، درصد ناهنجاری و درصد عملکرد غشای پلاسمایی اسپرم در زمان های مختلف اندازه گیری شد. تجزیه و تحلیل داده‌ها با استفاده از رویه‌ GLM و نرم افزار SAS 9.4 انجام شد. در مطالعه حاضر، افزودن سلنیوم به جیره در سطح 0/30 میلی‌گرم در کیلوگرم، صرف نظر از منبع آن، موجب افزایش غلظت، درصد جنبایی، زنده‌مانی و عملکرد غشای پلاسمایی اسپرم و کاهش درصد ناهنجاری اسپرم شد. در این مطالعه تغییری در حجم منی با افزودن سلنیوم به جیره خروس‌های مسن مشاهده نشد همچنین غلظت، جنبانی، زندمانی و عملکرد غشای پلاسمایی اسپرم، در روز 40 آزمایش در خروس‌های دریافت کننده 0/30 میلی‌گرم در کیلوگرم سلنیوم از منابع سلنیوم آلی و نانو بایوکیلات سلنیوم به‌طور معنی‌داری بیش تر از سایر زمان ها بود. نتایج نشان داد که شکل آلی سلنیوم (منابع سلمکس و نانوبایوکیلات سلنیوم) نسبت به شکل معدنی (سلنیت سدیم)، در سطح 0/30 میلی‌گرم در کیلوگرم به جیره باعث بهبود عملکرد تولیدمثلی خروس‌های مسن می شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effects of different selenium sources on sperm quality parameters in aged broiler breeder roosters

نویسندگان [English]

  • Morteza Asghari Moghadam 1
  • Seyed Reza Hashemi 1
  • Mehran Mehri 2
  • Amir Karamzadeh Dehaghani 3
  • Homa Davoodi 4
1 Department of Animal Science, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Department of Animal Science, Faculty of Agricalture, Zabol University, Zabol, Iran
3 Department of Animal Science, Faculty of Agricalture, University of Tehran, Karaj, Iran
4 Department of Immunology, Faculty of Medical Science, Golestan University of Medical Science, Gorgan, Iran
چکیده [English]

In order to improve fertility, selenium supplementation serves as an important consideration in animals' diets. The impacts of selenium supplementation depend on the source and quantity of selenium in the diet, so this study was performed to evaluate the effects of different selenium sources on sperm quality parameters in aged broiler breeder roosters (BBR). The study took place over a 40-day period; for the first two weeks, all birds received the same basal diet, and then for the next 40 days, the roosters were fed the experimental diets containing different sources (i.e., sodium selenite, organic selenium, and selenium nano-bio-chelate) and levels of selenium (i.e., 0, 0.15, 0.30, and 0.45 mg/kg). Every ten days, sperm was collected from the BBR by the belly rub method. The collected sperm was evaluated for several qualitative parameters such as volume, concentration, viability, and plasma membrane function. Each parameter was assessed using the General Linear Model (GLM) procedure of SAS 9.4 software. The results of the present study showed that supplementing the diet with selenium at a level of 0.30 mg/kg, regardless of its source, led to an increase in the concentration and motility percentage of sperm, as well as a decrease in the percentage of abnormal sperm. Furthermore, the plasma membrane function of sperm was also enhanced following the addition of selenium to the diet. These findings suggest that selenium supplementation, particularly at a level of 0.30 mg/kg, may improve sperm quality parameters and promote fertility in aged BBR. The present study indicated that the dietary supplementation of selenium, particularly at a level of 0.30 mg/kg, has a positive impact on the reproductive performance of old roosters. The results revealed that the organic form of selenium, such as Selmax and selenium nano-bio-chelate were more effective than sodium selenite, a popular inorganic form of selenium, in enhancing the fertility of the roosters.

کلیدواژه‌ها [English]

  • Rooster
  • nano-bio
  • selenium
  • sperm
  • fertility

مراجع

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فصلنامه محیط زیست جانوری
دوره 15، شماره 2
مرداد 1402
صفحه 149-156

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