“Functional glycomics”is a coming area of glycoscience and glycotechnology, which aims at elucidating biological roles of protein glycosylation from a holistic viewpoint, in a particular context of post-genomics and post-proteomics. However, analysis of glycans, i.e., the third bio-informative chain, should meet various difficulties due to many factors which other bio-macromolecules lack, e.g., branching and linkage isomerism. Recently, unique methods, which enable a large scale of identification methods of glycoproteins have been developed independently in two groups. In this chapter, advanced methods for glycoproteomics enabling identification of both core proteins and glycosylation sites are described as well as subsequent glycan profiling by means of lectin-affinity technologies.