Cytochrome c oxidase subunit I gene in Thalassiosira nordenskioeldii strains inhabiting in cold and warm sea waters

Yoshie UCHIDA; Hidenobu UCHIDA; Takeshi SATO; Yuko NISHIMOTO; Koichi TSUTSUMI; Takao OI; Mitsutaka TANIGUCHI; Kazuhito INOUE; Yoshihiro SUZUKI

doi:10.2183/pjab.100.010

Original Articles

Cytochrome c oxidase subunit I gene in Thalassiosira nordenskioeldii strains inhabiting in cold and warm sea waters

Yoshie UCHIDA, Hidenobu UCHIDA , Takeshi SATO, Yuko NISHIMOTO, Koichi TSUTSUMI, Takao OI, Mitsutaka TANIGUCHI, Kazuhito INOUE, Yoshihiro SUZUKI

Author information

Yoshie UCHIDA
https://orcid.org/0009-0009-1120-6489
Department of Food Business, School of Health and Human Life, Nagoya Bunri University
Hidenobu UCHIDA Corresponding author
https://orcid.org/0000-0002-7714-0402
Department of Food Business, School of Health and Human Life, Nagoya Bunri University
Research Institute for Integrated Science, Kanagawa University
Takeshi SATO
https://orcid.org/0009-0000-4327-5678
Research Institute for Integrated Science, Kanagawa University
Department of Science, Faculty of Science, Kanagawa University
Yuko NISHIMOTO
https://orcid.org/0000-0002-5437-8952
Department of Science, Faculty of Science, Kanagawa University
Koichi TSUTSUMI
Department of Food Business, School of Health and Human Life, Nagoya Bunri University
Takao OI
https://orcid.org/0000-0001-6664-849X
Graduate School of Bioagricultural Sciences, Nagoya University
Mitsutaka TANIGUCHI
https://orcid.org/0000-0001-6485-2462
Graduate School of Bioagricultural Sciences, Nagoya University
Kazuhito INOUE
https://orcid.org/0000-0002-5069-5280
Research Institute for Integrated Science, Kanagawa University
Department of Biochemistry and Biotechnology, Faculty of Chemistry and Biochemistry, Kanagawa University
Yoshihiro SUZUKI
Research Institute for Integrated Science, Kanagawa University
Department of Science, Faculty of Science, Kanagawa University

Keywords: cold water culturable strain, cytochrome c oxidase subunit I gene (COI), ice alga, intron, mitochondrial genome, Thalassiosira nordenskioeldii

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Supplementary material

2024 Volume 100 Issue 2 Pages 140-148

DOI https://doi.org/10.2183/pjab.100.010

Details

Abstract

From the biota beneath the sea ice in Lake Saroma, which is adjacent to Sea of Okhotsk, a diatom culture of Saroma 16 was isolated. Strutted processes and a labiate process in Saroma 16 were characteristic of those in Thalassiosira nordenskioeldii. Similarity search analysis showed that the 826-bp rbcL-3P region sequence of this strain was 100% identical to multiple sequences registered as T. nordenskioeldii in a public database. The 4305-bp PCR-amplified mitochondrial cytochrome c oxidase subunit I (COI) gene (COI)-5P region of Saroma 16 included a 1060-bp open reading frame (ORF), which was interrupted by 934-bp and 2311-bp introns that included frame-shifted ORFs encoding reverse-transcriptase (RTase)-like proteins. Previous reports showed that a strain of the same species, CNS00052, originating from the East China Sea included no introns in the COI, whereas North Atlantic Ocean strains of the same species, such as CCMP992, CCMP993, and CCMP997, included a 2.3-kb intron in the same position as Saroma 16.

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