Efek Asam Alfa Lipoat pada Kadar MDA dan Histologi Ginjal Tikus Wistar Diabetes Melitus Tipe1

Authors

  • Novita Fahrianti Putri Universitas Brawijaya
  • Diana Lyrawati Laboratorium Farmasi Fakultas Kedokteran Universitas Brawijaya Malang
  • Imam Sarwono Universitas Brawijaya, Rumah sakit Umum Dr. Saiful Anwar Malang

DOI:

https://doi.org/10.21776/ub.jkb.2015.028.03.3

Abstract

Asam alfa lipoat (ALA) telah banyak digunakan dalam pencegahan dan mengatasi komplikasi diabetes akibat spesies oksigen reaktif (ROS). Penelitian ini bertujuan untuk membuktikan bahwa ALA dapat mencegah peningkatan stres oksidatif pada ginjal tikus diabetes mellitus (DM) tipe 1. Penelitian eksperimental menggunakan Post Test Only Control Group dilakukan terhadap 30 tikus Wistar jantan. Sampel dipilih secara acak ke dalam kelompok normal (NTA), DM (DTA), DM dengan terapi per oral ALA 80 (DA80), 200 (DA200), dan 500 (DA500) mg/kg berat badan/hari. Variabel yang diukur pada penelitian ini adalah kadar malondialdehid (MDA) yang diukur dengan spektrofotometri, bobot dan histologi ginjal dengan pewarnaan hematoksilin dan eosin. Hasil penelitian menunjukkan bahwa kadar malondialdehid tertinggi ialah pada kelompok DA80 (45,500 ± 9,4538; p=0,870) dan rasio bobot ginjal/berat badan tertinggi ialah pada kelompok DA200 (0,013 ± 0,0060; p =0,537) namun secara statistika hasilnya tidak bermakna. Pengamatan histologi pada glomerulus ginjal menunjukkan adanya kerusakan (pembentukan stroma dan pengecilan ukuran inti sel) pada kelompok DTA, DA80, DA200, dan DA500. Kesimpulan dari penelitian ini adalah pemberian ALA per oral dalam waktu empat minggu tidak bisa menurunkan stres oksidatif maupun memperbaiki histologi ginjal pada DMT1, bahkan memperburuk gambaran histologi ginjal.

Kata Kunci: Asam alfa lipoat, DM1,histologi ginjal, malondialdehid

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Author Biographies

Novita Fahrianti Putri, Universitas Brawijaya

Program Studi Farmasi

Diana Lyrawati, Laboratorium Farmasi Fakultas Kedokteran Universitas Brawijaya Malang

Laboratory of Pharmacy

Imam Sarwono, Universitas Brawijaya, Rumah sakit Umum Dr. Saiful Anwar Malang

Laboratorium Patologi Anatomi

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Published

2015-02-13

Issue

Vol. 28 No. 3 (2015)

Section

Research Article

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