Augementation of macrophage-mediated tumor cell cytotoxicity by methyl vitamin B12

Hiroko Tsutsui; Yasuhiro Mizoguchi; Hiroko Katoh; Keiji Miyajima; Takayuki Arai; Yoshihide Sakagami; Toshihiro Higashimori; Sukeo Yamamoto; Seiji Morisawa

doi:10.2177/jsci.7.197

Augementation of macrophage-mediated tumor cell cytotoxicity by methyl vitamin B₁₂

Hiroko Tsutsui, Yasuhiro Mizoguchi, Hiroko Katoh, Keiji Miyajima, Takayuki Arai, Yoshihide Sakagami, Toshihiro Higashimori, Sukeo Yamamoto, Seiji Morisawa

Author information

Hiroko Tsutsui
The Third Department of Internal Medicine, Osaka City University, Medical School
Yasuhiro Mizoguchi
The Third Department of Internal Medicine, Osaka City University, Medical School
Hiroko Katoh
The Third Department of Internal Medicine, Osaka City University, Medical School
Keiji Miyajima
The Third Department of Internal Medicine, Osaka City University, Medical School
Takayuki Arai
The Third Department of Internal Medicine, Osaka City University, Medical School
Yoshihide Sakagami
The Third Department of Internal Medicine, Osaka City University, Medical School
Toshihiro Higashimori
The Third Department of Internal Medicine, Osaka City University, Medical School
Sukeo Yamamoto
The Third Department of Internal Medicine, Osaka City University, Medical School
Seiji Morisawa
The First Department of Biochemistry, Osaka City University, Medical School

Keywords: macrophage-mediated cytotoxicity, lipopolysaccharide

JOURNAL FREE ACCESS

1984 Volume 7 Issue 4 Pages 197-201

DOI https://doi.org/10.2177/jsci.7.197

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Abstract

Peritoneal macrophages become cytotoxic for the tumor cells by stimulating with lipopolysaccharide prepared from a Gram-negative bacteria. Although these cytotoxic action on tumor cells was not demonstrable when macrophages were treated with lower dosis of LPS than 1μg/ml, simultaneous addition of methyl-vitamin B₁₂ with LPS resulted in a significant augmentation of tumor cell cytotoxicity. Since vitamin B₁₂ alone did not induce any cytotoxic macrophages, vitamin B₁₂ may increase in the actvation of macrophages partially stimulated with low dosis LPS.

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