Technetium-99m-labeled Recombinant Tissue Plasminogen Activator for the Imaging of Emboli In Vivo

Akihiro TAKAHASHI; Kazuo ITOH; Eriko TSUKAMOTO; Masayori FURUDATE; Hiroyasu KAMIYAMA; Hiroshi ABE

doi:10.2176/nmc.33.415

Akihiro TAKAHASHI, Kazuo ITOH, Eriko TSUKAMOTO, Masayori FURUDATE, Hiroyasu KAMIYAMA, Hiroshi ABE

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Keywords: recombinant tissue-type plasminogen activator, technetium, thrombus imaging, cerebral embolism

JOURNAL FREE ACCESS

1993 Volume 33 Issue 7 Pages 415-419

DOI https://doi.org/10.2176/nmc.33.415

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Abstract

Tissue-type plasminogen activator (t-PA) effectively lyses active thrombus by direct action. Recombinant t-PA (rt-PA) was labeled with technetium-99m (^99mTc) to investigate the in vivo binding to fibrin clots in a feline cerebral embolism model created by insertion of an artificial fibrin clot within the carotid artery. ^99mTc-rt-PA administered intravenously provided clearer imaging of clots after priming with cold rt-PA, with uptake peaking 5-10 minutes after the injection. ^99mTc-labeled human serum albumin was not retained at clot sites. Systemically administered ^99mTc-rt-PA binds to fibrin clots within carotid arteries in our feline model. Our results suggest that the interaction of intrinsic plasminogen activator inhibitors with extrinsically administered rt-PA may regulate the demonstration of a clot, although the precise mechanism is unclear.

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