Solid state radiation chemistry of the DNA backbone
The long term goal of this program is to determine the fundamental rules needed to predict the type and yield of damage produced in DNA due to direct effects of ionizing radiation. The focus is on damage to the sugar-phosphate backbone, damage that would lead to strand breaks. Model systems have been chosen that permit various aspects of this problem to be investigated. The emphasis will be on single crystals of monosaccharides, nucleosides, and nucleotides but will also include some powder work on polynucleotides. In these model systems, free radical products and reactions are observed by electron spin resonance (ESR) and electron nuclear double resonance (ENDOR) techniques. The information thus gained is used in constructing rules that predict what primary free radicals are formed in single crystals of model compounds and the reactions stemming from the primary radicals. The formulation of a set of rules that work in model systems will represent a major advance toward formulating a set of rules that predict the direct damage in DNA itself. In a broader context this program is part of the effort to understand and predict the effects of exposure to ionizing radiation received at low dose rates over long periods of time. Assessment of low dose effects requires a basic understanding of the action of radiation at the molecular level. By contributing to that basic understanding, this program will help solve the problems of risk assessment under low dose conditions. 5 refs., 3 figs.
- Research Organization:
- Univ. of Rochester, NY (United States)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG02-88ER60625
- OSTI ID:
- 5430309
- Report Number(s):
- DOE/ER/60625-1; ON: DE90003665; TRN: 89-031949
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
MATHEMATICAL MODELS
STRAND BREAKS
RADIOINDUCTION
IONIZING RADIATIONS
MONOSACCHARIDES
PROGRESS REPORT
RADIATION CHEMISTRY
CARBOHYDRATES
CHEMISTRY
DOCUMENT TYPES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
SACCHARIDES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture