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Title: Reconfiguration of the Respiratory Tract Microbiome to Prevent and Treat Burkholderia Infection

Technical Report ·
DOI:https://doi.org/10.2172/1898252· OSTI ID:1898252
 [1];  [2];  [3];  [3];  [4];  [1];  [2];  [5];  [5];  [6];  [1];  [1];  [6];  [1];  [4]
  1. Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
  2. Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
  3. Georgia Institute of Technology, Atlanta, GA (United States)
  4. Sandia National Laboratories (SNL-CA), Livermore, CA (United States)
  5. Univ. of California, Berkeley, CA (United States)
  6. Sandia National Laboratories (SNL-CA), Livermore, CA (United States); Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
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New approaches to preventing and treating infections, particularly of the respiratory tract, are needed. One promising strategy is to reconfigure microbial communities (microbiomes) within the host to improve defense against pathogens. Probiotics and prebiotics for gastrointestinal (GI) infections offer a template for success. We sought to develop comparable countermeasures for respiratory infections. First, we characterized interactions between the airway microbiome and a biodefense-related respiratory pathogen (Burkholderia thailandensis; Bt), using a mouse model of infection. Then, we recovered microbiome constituents from the airway and assessed their ability to re-colonize the airway and protect against respiratory Bt infection. We found that microbiome constituents belonging to Bacillus and related genuses frequently displayed colonization and anti-Bt activity. Comparative growth requirement profiling of these Bacillus strains vs Bt enabled identification of candidate prebiotics. This work serves as proof of concept for airway probiotics, as well as a strong foundation for development of airway prebiotics.

Research Organization:
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
DOE Contract Number:
NA0003525
OSTI ID:
1898252
Report Number(s):
SAND2022-15184; 711829
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES