Sarcoidosis Associated with Enlarged Mediastinal Lymph Nodes with the Detection of Streptococcus gordonii and Cutibacterium acnes Using a Clone Library Method

Kentaro Akata; Kei Yamasaki; Kazuki Nemoto; Hiroaki Ikegami; Takako Kawaguchi; Shingo Noguchi; Toshinori Kawanami; Kazumasa Fukuda; Hiroshi Mukae; Kazuhiro Yatera

doi:10.2169/internalmedicine.1887-23

CASE REPORTS

Sarcoidosis Associated with Enlarged Mediastinal Lymph Nodes with the Detection of Streptococcus gordonii and Cutibacterium acnes Using a Clone Library Method

Kentaro Akata, Kei Yamasaki, Kazuki Nemoto, Hiroaki Ikegami, Takako Kawaguchi, Shingo Noguchi, Toshinori Kawanami, Kazumasa Fukuda, Hiroshi Mukae, Kazuhiro Yatera

Author information

Kentaro Akata
Division of Infection Control and Prevention, University of Occupational and Environmental Health, Japan
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Kei Yamasaki
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Kazuki Nemoto
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Hiroaki Ikegami
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Takako Kawaguchi
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Shingo Noguchi
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Toshinori Kawanami
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
Kazumasa Fukuda
Department of Microbiology, University of Occupational and Environmental Health, Japan
Hiroshi Mukae
Department of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Japan
Kazuhiro Yatera
Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan

Keywords: sarcoidosis, bacterial flora, 16S ribosomal RNA gene analysis

JOURNAL OPEN ACCESS

2024 Volume 63 Issue 2 Pages 299-304

DOI https://doi.org/10.2169/internalmedicine.1887-23

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Abstract

A 77-year-old Japanese woman with mediastinal lymphadenopathy and uveitis was diagnosed with sarcoidosis. The bacterial flora in biopsied samples from mediastinal lymph nodes was analyzed using a clone library method with Sanger sequencing of the 16S rRNA gene, and Streptococcus gordonii (52 of 71 clones) and Cutibacterium acnes (19 of 71 clones) were detected. No previous study has conducted a bacterial floral analysis using the Sanger method for the mediastinal lymph node in sarcoidosis, making this case report the first to document the presence of S. gordonii and C. acnes in the mediastinal lymph node of a patient with sarcoidosis.

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