Histological Effect of Diphenol-A in Eyes of Chicken Embryos

Almustafa, Sarab Shaher; Eid, Wafaa Sabri; Alsaffo, Alyaa Ali

doi:10.21608/ejvs.2024.273731.1884

Histological Effect of Diphenol-A in Eyes of Chicken Embryos

Document Type : Original Article

Authors

¹ Department of Basic Nursing Sciences, College of Nursing, University of Mosul

² Department of Pathology, College of Medicine, University of Mosul

³ Department of Anatomy, College of Medicine, University of Mosul

10.21608/ejvs.2024.273731.1884

Abstract

Diphenol-A (bisphenole-A) can leach into meals and beverages from containers containing Diphenol A. Diphenol A exposure is concerning due to the potential consequences on the brain and organs of fetuses, babies, and children. To be assess the teratological and histopathological changes in the eyes of chick embryos that exposure to Diphenol we used number of 150 fertilized chicken eggs divided into 6 groups (Diphenol given in the second day of incubation, injected in to the air sac). G1, Negative control group chicks embryos without any exposure. G2, Positive control treated with corn oil. G3, treated with 0.0125 μL /egg Diphenol. G4, 0.025 μL /egg Diphenol. G5, 0.05 μL /egg Diphenol. G6, 0.1 μL /egg Diphenol. The chick embryos was euthanized at a developmental stage of (21) day. The study examined eye tissues for histopathological changes, revealing a range of abnormalities in different groups in subsequent treatment groups (G3 to G6), pathological alterations were identified. Groups G3 to G6 showed blood vessels, hemorrhagic foci, moderate degeneration in nerve fiber layers, edema in the nuclear fiber layer, and severe degenerative changes in the lamina pectoris, fibrous layer cells, and rods and cones layer of the retina. The study concluded that Diphenol may have teratological and histological abnormalities in the eyes of chicken embryos and that warrant further investigation.

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