Dispersive Liqui-Liquid Microextraction Spectrophotometric Determination of Human Salivary Nitrite Content

Document Type : Original Article

Authors

Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil, Iraq,44002.

Abstract

novel and rapid dispersive liquid-liquid microextraction (DLLME) method was applied to intensify nitrite ions as a subsequent step to its spectrophotometric determination using the Griess reaction. Produced orange azo dye has (λ max.) of 460 nm., (ε) is 3.8432 x 103 L. mol-l.cm-1, and Beer’s law was obeyed in the range 0.3-12.0 µg. mL-1. It shows LOD, LOQ, and R2 values of 0.2 µg. mL-1, 0.3 µg. mL-1 and 0.9994, respectively. In the recommended DLLME technique, ethanol was used as the dispersant solvent and a minimal amount of chloroform was used as the extraction solvent. Then, the dye has been extracted into fine chloroform droplets. The solution was centrifuged for 3.0 min./5000 rpm, and the droplets sank to the bottom of a 10.0 mL conical test tube. The total amount of azo-dye enriched extracted phase was then measured using a spectrophotometer. The yellow chloroform extract exhibits (λ max.) of 410 nm, (ε) is 8.6024 x 103 L. mol-l.cm-1and Beer’s law was obeyed in the range 0.1-7.0 µg. mL-1. The LOD, LOQ, and R2 values of the proposed extraction method were 0.05 µg. mL-1, 0.1 µg. mL -1 and 0.9997, respectively. Sensitivity enhancement was around 225%. The proposed technique was successfully applied to human saliva samples.

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History

  • Receive Date: 16 September 2022
  • Revise Date: 19 October 2022
  • Accept Date: 22 November 2022

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