The oviducts from 150-day-old White Leghorn laying hens were homogenized, fractionated by differential centrifugation, and were incubated with the radioactive steroids. Incubation of progesterone with the oviduct tissue homogenate, produced 5α-pregnane-3, 20-dione, 5α-pregnan-3α, 20β-diol and 3α-hydroxy-5α-pregnan-20-one. Androstenedione was metabolized to 5β-androstane-3, 17-dione, 5β-androstane-3α, 17β-diol and 5β-dihydrotestosterone. Testosrone was metabolized to androstenedione, 5β-androstane-3, 17-dione and 5β-dihydrotesosterone. Estrone was metabolized to estradiol. Estradiol was metabolized to estrone, estriol and 16-epiestriol. The activities of Δ⁴-5α-hydrogenase upon progesterone, and 17β-hydroxysteroid dehydrogenase upon androstenedione, testosterone and estrone were evident in the supernatant and nuclear fractions. 17α-Hydroxylase and C_17-20lyase were not present in the tissue. Low activities of the steroidogenesis related enzymes in the microsomal fraction, and lacking in 17α-hydroxylase are characteistic of the target organs for steroid hormones such as ouiduct, brain14) and epididymis15).