Abdelhamid AF, Ayoub HF, Abd El-Gawad EA, Abdelghany MF, Abdel-Tawwab M (2021) Potential effects of dietary seaweeds mixture on the growth performance, antioxidant status, immunity response, and resistance of striped catfish (Pangasianodon hypophthalmus) against Aeromonas hydrophila infection. Fish & Shellfish Immunology, 119, 76-83. https://doi.org/10.1016/j.fsi.2021.09.043
Ansari FA, Guldhe A, Gupta SK, Rawat I, Bux F (2021) Improving the feasibility of aquaculture feed by using microalgae. Environmental Science and Pollution Research, 28(32), 43234-43257. https://doi.org/10.1007/s11356-021-14989-x
AOAC (1998) Officinal Methods of Analysis. 16th ed.. AOAC International. Gaithersburg. MD. USA.
Ashour M, Mabrouk MM, Abo-Taleb HA, Sharawy ZZ, Ayoub HF, Van Doan H, ..., Goda AMA (2021) A liquid seaweed extract (TAM®) improves aqueous rearing environment, diversity of zooplankton community, whilst enhancing growth and immune response of Nile tilapia, Oreochromis niloticus, challenged by Aeromonas hydrophila. Aquaculture, 543, 736915. https://doi.org/10.1016/j.aquaculture.2021.736915
Ashour M, Mabrouk MM, Ayoub HF, El-Feky MM, Zaki SZ, Hoseinifar SH, ..., Goda AMS (2020) Effect of dietary seaweed extract supplementation on growth, feed utilization, hematological indices, and non-specific immunity of Nile Tilapia, Oreochromis niloticus challenged with Aeromonas hydrophila. Journal of Applied Phycology, 32, 3467-3479. https://doi.org/10.1007/s10811-020-02178-1
Bahi, A., Ramos‐Vega, A., Angulo, C., Monreal‐Escalante, E., & Guardiola, F. A. (2023). Microalgae with immunomodulatory effects on fish. Reviews in Aquaculture. https://doi.org/10.1111/raq.12792
Basri NA, Shaleh SRM, Matanjun P, Noor NM, Shapawi R (2015) The potential of microalgae meal as an ingredient in the diets of early juvenile Pacific white shrimp, Litopenaeus vannamei. J Appl Phycol 27:857–863. https://doi.org/10.1007/s10811-014-0383-6
Batista S, Pereira R, Oliveira B, Baião LF, Jessen F, Tulli F, ..., Valente LM (2020) Exploring the potential of seaweed Gracilaria gracilis and microalga Nannochloropsis oceanica, single or blended, as natural dietary ingredients for European seabass Dicentrarchus labrax. Journal of Applied Phycology, 32, 2041-2059. https://doi.org/10.1007/s10811-020-02118-z
Bélanger A, Sarker PK, Bureau DP, Chouinard Y, Vandenberg GW (2021) Apparent digestibility of macronutrients and fatty acids from microalgae (Schizochytrium sp.) fed to rainbow trout (Oncorhynchus mykiss): A potential candidate for fish oil substitution. Animals. 11(2). 456. https://doi.org/10.3390/ani11020456
Bilen S, Altunoglu YC, Ulu F, Biswas G (2016) Innate immune and growth promoting responses to caper (Capparis spinosa) extract in rainbow trout (Oncorhynchus mykiss). Fish & shellfish immunology, 57, 206-212. https://doi.org/10.1016/j.fsi.2016.08.040
Bilen S, Ali GAM, Amhamed ID, Almabrok AA (2021) Modulatory effects of laurel-leaf cistus (Cistus laurifolius) ethanolic extract on innate immune responses and disease resistance in common carp (Cyprinus carpio). Fish & shellfish immunology. 116. 98-106. https://doi.org/10.1016/j.fsi.2021.07.001
Chen W, Luo L, Han D, Long F, Chi Q, Hu Q (2021) Effect of dietary supplementation with Chlorella sorokiniana meal on the growth performance, antioxidant status, and immune response of rainbow trout (Oncorhynchus mykiss). Journal of Applied Phycology, 33, 3113-3122. https://doi.org/10.1007/s10811-021-02541-w
Dineshbabu G, Goswami G, Kumar R, Sinha A, Das D (2019) Microalgae–nutritious, sustainable aqua-and animal feed source. Journal of Functional Foods, 62, 103545. https://doi.org/10.1016/j.jff.2019.103545
Farag MR, Alagawany M, Khalil SR, Abd El-Aziz RM, Zaglool AW, Moselhy AA, Abou-Zeid SM (2022) Effect of parsley essential oil on digestive enzymes, intestinal morphometry, blood chemistry and stress-related genes in liver of Nile tilapia fish exposed to Bifenthrin. Aquaculture, 546, 737322. https://doi.org/10.1016/j.aquaculture.2021.737322
Ferreira M, Teixeira C, Abreu H, Silva J, Costas B, Kiron V, Valente LM (2021) Nutritional value, antimicrobial and antioxidant activities of micro-and macroalgae, single or blended, unravel their potential use for aquafeeds. Journal of Applied Phycology, 33(6), 3507-3518. https://doi.org/10.1007/s10811-021-02549-2
García JL, De Vicente M, Galán B (2017) Microalgae, old sustainable food and fashion nutraceuticals. Microbial biotechnology, 10(5), 1017. https://doi.org/10.1111/1751-7915.12800
García-Beltrán JM, Mansour AT, Alsaqufi AS, Ali HM, Esteban MÁ (2020) Effects of aqueous and ethanolic leaf extracts from drumstick tree (Moringa oleifera) on gilthead seabream (Sparus aurata L.) leucocytes, and their cytotoxic, antitumor, bactericidal and antioxidant activities. Fish & Shellfish Immunology, 106, 44-55. https://doi.org/10.1016/j.fsi.2020.06.054
Habte-Tsion HM, Kolimadu GD, Rossi Jr W, Filer K, Kumar V (2020) Effects of Schizochytrium and micro-minerals on immune, antioxidant, inflammatory and lipid-metabolism status of Micropterus salmoides fed high-and low-fishmeal diets. Scientific Reports, 10(1), 7457. https://doi.org/10.1038/s41598-020-64286-9
Hamidi M, Kozani PS, Kozani PS, Pierre G, Michaud P, Delattre C (2019) Marine bacteria versus microalgae: who is the best for biotechnological production of bioactive compounds with antioxidant properties and other biological applications?. Marine drugs, 18(1), 28. https://doi.org/10.3390/md18010028
Hemaiswarya, S., Raja, R., Ravi Kumar, R., Ganesan, V., & Anbazhagan, C. (2011). Microalgae: a sustainable feed source for aquaculture. World Journal of Microbiology and Biotechnology, 27, 1737-1746.
Hernández-Contreras, Á., Teles, A., Salas-Leiva, J. S., Chaves-Pozo, E., & Tovar-Ramírez, D. (2023). Feed Additives in Aquaculture. In Sustainable Use of Feed Additives in Livestock: Novel Ways for Animal Production (pp. 811-846). Cham: Springer International Publishing.
Hoseinifar SH, Fazelan Z, Bayani M, Yousefi M, Van Doan H, Yazici M (2022). Dietary red macroalgae (Halopithys incurva) improved systemic an mucosal immune and antioxidant parameters and modulated related gene expression in zebrafish (Danio rerio). Fish & Shellfish Immunology, 123, 164-171. https://doi.org/10.1016/j.fsi.2022.02.047
Hoseinifar SH, Shakouri M, Van Doan H, Shafiei S, Yousefi M, Raeisi M, ..., Reverter M (2020) Dietary supplementation of lemon verbena (Aloysia citrodora) improved immunity, immune-related genes expression and antioxidant enzymes in rainbow trout (Oncorrhyncus mykiss). Fish & shellfish immunology, 99, 379-385. https://doi.org/10.1016/j.fsi.2020.02.006
Hoseinifar SH, Yousefi S, Capillo G, Paknejad H, Khalili M, Tabarraei A, ..., Faggio C (2018) Mucosal immune parameters, immune and antioxidant defence related genes expression and growth performance of zebrafish (Danio rerio) fed on Gracilaria gracilis powder. Fish & shellfish immunology, 83, 232-237. https://doi.org/10.1016/j.fsi.2018.09.046
Jo Rivero CL (2021) Effects of three different microalgae on growth performances and immune responses in juvenile rainbow trout. Oncorhynchus mykiss. Master of Fisheries Science. Division of Fisheries Science. The Graduate School of World Fisheries University. Pukyong National University Busan, South Korea.
Karapanagiotidis IT, Metsoviti MN, Gkalogianni EZ, Psofakis P, Asimaki A, Katsoulas N, ..., Zarkadas I (2022). The effects of replacing fishmeal by Chlorella vulgaris and fish oil by Schizochytrium sp. and Microchloropsis gaditana blend on growth performance, feed efficiency, muscle fatty acid composition and liver histology of gilthead seabream (Sparus aurata). Aquaculture, 561, 738709. https://doi.org/10.1016/j.aquaculture.2022.738709
Karataş B (2019) A Research on the Evaluation and Management Of Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1792) in Van Province According to Growth Hormone (GH) Gene Expression Levels, Genetic Polymorphism and Some Phenotypic Parameters. PhD Thesis Aquaculture Engineering. Van Yüzüncü Yıl University, Institute of Science and Technology. Van, Türkiye.
Kiadaliri M, Firouzbakhsh F, Deldar H (2020) Effects of feeding with red algae (Laurencia caspica) hydroalcoholic extract on antioxidant defense, immune responses, and immune gene expression of kidney in rainbow trout (Oncorhynchus mykiss) infected with Aeromonas hydrophila. Aquaculture, 526, 735361. https://doi.org/10.1016/j.aquaculture.2020.735361
Komarek R J, Komarek AR, & Layton B (2004) Evaluation of the rapid. hightemperature extraction of feeds. foods. and oilseeds by the ANKOM XT20 fat analyzer to determine crude fat content. Oil Extraction and Analysis (Editor: Luthria. D.L.). AOCS Publishing. New York. 288.
Kotrbáček V, Doubek J, Doucha J (2015) The chlorococcalean alga Chlorella in animal nutrition: a review. Journal of Applied Phycology, 27, 2173-2180. https://doi.org/10.1007/s10811-014-0516-y
Kousoulaki K, Mørkøre T, Nengas I, Berge RK, Sweetman J (2016) Microalgae and organic minerals enhance lipid retention efficiency and fillet quality in Atlantic salmon (Salmo salar L.). Aquaculture, 451, 47-57. https://doi.org/10.1016/j.aquaculture.2015.08.027
Kousoulaki K, Sveen L, Norén F, Espmark Å (2022) Atlantic salmon (Salmo salar) performance fed low trophic ingredients in a fish meal and fish oil free diet. Frontiers in Physiology, 13, 884740. https://doi.org/10.3389/fphys.2022.884740
Lee S, Park CO, Choi W, Bae J, Kim J, Choi S, ..., Bai SC (2022) Partial substitution of fish oil with microalgae (Schizochytrium sp.) can improve growth performance. nonspecific immunity and disease resistance in rainbow trout. Oncorhynchus mykiss. Animals. 12(9). 1220. https://doi.org/10.3390/ani12091220
Li MH, Robinson EH, Tucker CS, Manning BB, Khoo L (2009) Effects of dried algae Schizochytrium sp., a rich source of docosahexaenoic acid, on growth, fatty acid composition, and sensory quality of channel catfish Ictalurus punctatus. Aquaculture, 292(3-4), 232-236. https://doi.org/10.1016/j.aquaculture.2009.04.033
Li Y, Le Q, Zhang M, Xu S, He S, Yan X, ..., Wang Y (2023) The Effect of Schizochytrium sp. on Growth, Fatty Acid Profile and Gut Microbiota of Silver Pomfret (Pampus argenteus). Journal of Marine Science and Engineering, 11(2), 414. https://doi.org/10.3390/jmse11020414
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods, 25(4), 402-408. https://doi.org/10.1006/meth.2001.1262
Luo Z, Ye HM, Gao Y, Ling SC, Wei CC, Zhu X (2018) Chlorella additive increased growth performance, improved appetite and immune response of juvenile crucian carp Carassius auratus. Aquaculture Research, 49(10), 3329-3337 https://doi.org/10.1111/are.13797
Ma K, Chen S, Wu Y, Ma Y, Qiao H, Fan J, Wu H (2022) Dietary supplementation with microalgae enhances the zebrafish growth performance by modulating immune status and gut microbiota. Applied Microbiology and Biotechnology, 106(2), 773-788. https://doi.org/10.1007/s00253-021-11751-8
Morabito C, Bournaud C, Maës C, Schuler M, Cigliano RA, Dellero Y, ..., Rébeillé F (2019) The lipid metabolism in thraustochytrids. Progress in Lipid Research, 76, 101007. https://doi.org/10.1016/j.plipres.2019.101007
Mota CS, Pinto O, Sá T, Ferreira M, Delerue-Matos C, Cabrita AR, ..., Maia MR (2023) A commercial blend of macroalgae and microalgae promotes digestibility, growth performance, and muscle nutritional value of European seabass (Dicentrarchus labrax L.) juveniles. Frontiers in Nutrition, 10, 1165343. https://doi.org/10.3389/fnut.2023.1165343
Mousavi S, Sheikhzadeh N, Tayefi-Nasrabadi H, Alizadeh-Salteh S, Khani Oushani A, Firouzamandi M, Mardani K (2020) Administration of grape (Vitis vinifera) seed extract to rainbow trout (Oncorhynchus mykiss) modulates growth performance, some biochemical parameters, and antioxidant-relevant gene expression. Fish physiology and biochemistry, 46(3), 777-786. https://doi.org/10.1007/s10695-019-00716-4
Naderi M, Keyvanshokooh S, Salati AP, Ghaedi A, (2017) Effects of chronic high stocking density on liver proteome of rainbow trout (Oncorhynchus mykiss). Fish. Physiol. Biochem. 43 (5), 1373–1385. https://doi.org/10.1007/s10695-017-0378-8.
Nagappan, S., Das, P., AbdulQuadir, M., Thaher, M., Khan, S., Mahata, C., ... & Kumar, G. (2021). Potential of microalgae as a sustainable feed ingredient for aquaculture. Journal of Biotechnology, 341, 1-20. https://doi.org/10.1016/j.jbiotec.2021.09.003
Önalan Ş (2019) Expression differences of stress and immunity genes in rainbow trout (Oncorhynchus mykiss, Walbaum 1792) with different bacterial fish diseases. Israeli Journal of Aquaculture-Bamidgeh, 71.
Peixoto MJ, Magnoni L, Gonçalves JF, Twijnstra RH, Kijjoa A, Pereira R, ..., Ozório RO (2019) Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax). Journal of applied phycology, 31, 761-770. https://doi.org/10.1007/s10811-018-1519-x
Peixoto MJ, Salas-Leitón E, Pereira LF, Queiroz A, Magalhães F, Pereira R, ..., de Almeida Ozório RO (2016a) Role of dietary seaweed supplementation on growth performance, digestive capacity and immune and stress responsiveness in European seabass (Dicentrarchus labrax). Aquaculture Reports, 3, 189-197. https://doi.org/10.1016/j.aqrep.2016.03.005
Peixoto MJ, Svendsen JC, Malte H, Pereira LF, Carvalho P, Pereira R, ..., Ozório RO (2016b) Diets supplemented with seaweed affect metabolic rate, innate immune, and antioxidant responses, but not individual growth rate in European seabass (Dicentrarchus labrax). Journal of Applied Phycology, 28, 2061-2071. https://doi.org/10.1007/s10811-015-0736-9
Peng K, Chen X, Wei D, Zhao L, Chen B, Mo W, ..., Sun Y (2020) Inclusion of Chlorella water extract in Oreochromis niloticus fingerling diets: Effects on growth performance, body composition, digestive enzyme activity, antioxidant and immune capacity, intestine and hepatic histomorphology and sodium nitrite stress resistance. Aquaculture Reports, 18, 100547. https://doi.org/10.1016/j.aqrep.2020.100547
Quico CA, Astocondor MM, Ortega RA (2021) Dietary supplementation with Chlorella peruviana improve the growth and innate immune response of rainbow trout Oncorhynchus mykiss fingerlings. Aquaculture. 533. 736117. https://doi.org/10.1016/j.aquaculture.2020.736117
Radhakrishnan S, Seenivasan C, Muralisankar T (2015) Effect of dietary replacement of fishmeal with Chlorella vulgaris on growth performance, energy utilization and digestive enzymes in Macrobrachium rosenbergii postlarvae. International journal of Fisheries and Aquaculture, 7(5), 62-70. https://doi.org/10.5897/IJFA15.0471
Safavi SV, Kenari AA, Tabarsa M, Esmaeili M (2019) Effect of sulfated polysaccharides extracted from marine macroalgae (Ulva intestinalis and Gracilariopsis persica) on growth performance, fatty acid profile, and immune response of rainbow trout (Oncorhynchus mykiss). Journal of Applied Phycology, 31, 4021-4035. https://doi.org/10.1007/s10811-019-01902-w
Sajina KA, Sahu NP, Varghese T, Jain KK (2019) Fucoidan-rich Sargassum wightii extract supplemented with α-amylase improve growth and immune responses of Labeo rohita (Hamilton, 1822) fingerlings. Journal of Applied Phycology, 31, 2469-2480. https://doi.org/10.1007/s10811-019-1742-0
Sattanathan G, Liu WC, Padmapriya S, Pushparaj K, Sureshkumar S, Lee JW, ..., Kim IH (2022) Effects of Dietary Blend of Algae Extract Supplementation on Growth, Biochemical, Haemato-Immunological Response, and Immune Gene Expression in Labeo rohita with Aeromonas hydrophila Post-Challenges. Fishes, 8(1), 7. https://doi.org/10.3390/fishes8010007
Saurabh S, Sahoo PK (2008) Lysozyme: an important defence molecule of fish innate immune system. Aquaculture research, 39(3), 223-239. https://doi.org/10.1111/j.1365-2109.2007.01883.x
Serrano E, Simpfendorfer R, Medina A, Sandoval C, Martínez A, Morales R, Davies SJ (2021) Partially replacing fish oil with microalgae (Schizochytrium limacinum and Nannochloropsis oceanica) in diets for rainbow trout (Oncorhynchus mykiss) reared in saltwater with reference to growth performance, muscle fatty acid composition and liver ultrastructure. Aquaculture Research, 52(9), 4401-4413. https://doi.org/10.1111/are.15279
Shah MR, Lutzu GA, Alam A, Sarker P, Chowdhury K, Parsaeimehr A, ..., Daroch M (2018) Microalgae in aquafeeds for a sustainable aquaculture industry. Journal of applied phycology, 30(1), 197-213. https://doi.org/10.1007/s10811-017-1234-z
Sharawy ZZ, Ashour M, Abbas E, Ashry O, Helal M, Nazmi H, ..., Goda A (2020) Effects of dietary marine microalgae, Tetraselmis suecica, on production, gene expression, protein markers and bacterial count of Pacific white shrimp Litopenaeus vannamei. Aquaculture Research, 51(6), 2216-2228. https://doi.org/10.1111/are.14566
Shi Y, Cao X, Ye Z, Xu Y, Wang Y, Li Z, ..., He N (2021) Role of dietary Schizochytrium sp. in improving disease resistance of zebrafish through metabolic and microbial analysis. Aquaculture, 539, 736631. https://doi.org/10.1016/j.aquaculture.2021.736631
Sijil, P. V., Cherita, C., Jethani, H., & Chauhan, V. S. (2022). Microalgae as a Renewable and Sustainable Source of High Value Metabolites. In Microalgae for Sustainable Products: The Green Synthetic Biology Platform, ed. A. Shekh and S. Dasgupta, The Royal Society of Chemistry, 2022, ch. 1, pp. 1-26. https://doi.org/10.1039/9781839167508
Singh ST, Kamilya D, Kheti B, Bordoloi B, Parhi J (2017) Paraprobiotic preparation from Bacillus amyloliquefaciens FPTB16 modulates immune response and immune relevant gene expression in Catla catla (Hamilton, 1822). Fish & Shellfish Immunology, 66, 35-42. https://doi.org/10.1016/j.fsi.2017.05.005
Skalli A, Firmino JP, Andree KB, Salomón R, Estévez A, Puig P, Sabater-Martínez M, Hechavarria T, Gisbert E (2020) The inclusion of the microalga scenedesmus sp. In diets for rainbow trout, Onchorhynchus mykiss, juveniles. Animals, 10(9). https://doi.org/10.3390/ani10091656
Sørensen M, Berge GM, Reitan KI, Ruyter B (2016) Microalga Phaeodactylum tricornutum in feed for the Atlantic salmon (Salmo salar) - effect on nutrient digestibility, growth and utilization of feed. Aquaculture 460:116–123 https://doi.org/10.1016/j.aquaculture.2016.04.010
Souza FPD, Lima ECSD, Urrea-Rojas AM, Suphoronski SA, Facimoto CT, Bezerra Junior JDS, ..., Lopera-Barrero NM (2020) Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages. PloS one, 15(1), e0226977. https://doi.org/10.1371/journal.pone.0226977
Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101(2):87–96 https://doi.org/10.1263/jbb.101.87
Teimouri M, Yeganeh S, Mianji GR, Najafi M, Mahjoub S (2019) The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss). Fish physiology and biochemistry, 45(3), 977-986. https://doi.org/10.1007/s10695-019-0608-3
Valente LMP, Custódio M, Batista S, Fernandes H, Kiron V (2019) Defatted microalgae (Nannochloropsis sp.) from biorefinery as a potential feed protein source to replace fishmeal in European sea bass diets. Fish physiology and biochemistry, 45(3), 1067-1081. https://doi.org/10.1007/s10695-019-00621-w
Vazirzadeh A, Marhamati A, Rabiee R, Faggio C (2020) Immunomodulation, antioxidant enhancement and immune genes up-regulation in rainbow trout (Oncorhynchus mykiss) fed on seaweeds included diets. Fish & Shellfish Immunology, 106, 852-858. https://doi.org/10.1016/j.fsi.2020.08.048
Vizcaíno AJ, López G, Sáez MI, Jiménez JA, Barros A, Hidalgo L, ..., Alarcón FJ (2014) Effects of the microalga Scenedesmus almeriensis as fishmeal alternative in diets for gilthead sea bream, Sparus aurata, juveniles. Aquaculture, 431, 34-43. https://doi.org/10.1016/j.aquaculture.2014.05.010
Wang Y, Li M, Filer K, Xue Y, Ai Q, Mai K (2017) Replacement of fish oil with a DHA‐rich Schizochytrium meal on growth performance, activities of digestive enzyme and fatty acid profile of Pacific white shrimp (Litopenaeus vannamei) larvae. Aquaculture Nutrition, 23(5), 1113-1120. https://doi.org/10.1111/anu.12479
Wang Y, Li M, Lin G, Guo X, Sun A, Dong H, ..., Mai K (2022). Effects of Dietary Supplement of Schizochytrium Meal on Growth, Fatty Acid Profile and Activities of Digestive Enzymes in Turbot (Scophthalmus maximus L.) Larvae. Pakistan J. Zool., pp 1-10, https://dx.doi.org/10.17582/journal.pjz/20220401080451
Wangkahart E, Wachiraamonloed S, Lee PT, Subramani PA, Qi Z, Wang B (2022) Impacts of Aegle marmelos fruit extract as a medicinal herb on growth performance, antioxidant and immune responses, digestive enzymes, and disease resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 120, 402-410. https://doi.org/10.1016/j.fsi.2021.11.015
Yazdanpanah M, Sotoudeh E, Mansouri Taee H, Habibi H (2021) Dietary administration of Sargassum angustifolium and Gracilaria pulvinata extracts affect antioxidant enzyme activities and Lactobacillus bacterial population in intestine of rainbow trout (Oncorhynchus mykiss) fry. Iranian Journal of Fisheries Sciences, 20(4), 926-944. https:// doi.org/10.22092/ijfs.2021.124372