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Effects of chronic high stocking density on liver proteome of rainbow trout (Oncorhynchus mykiss)

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Abstract

The main aim of the present study was to assess the effects of chronic high stocking density on liver proteome of rainbow trout. Rainbow trout juveniles (42.6 ± 2.3 g average body weight) were randomly distributed into six tanks at two stocking densities (low stocking density (LD) = 20 kg m−3 and high stocking density (HD) = 80 kg m−3). Both treatments were performed in triplicate tanks for a period of 60 days. High stocking density caused a reduction in the growth performance compared with LD fish. Lysozyme activity increased with stocking density, while serum complement activity presented the opposite pattern. Serum cortisol and total protein levels did not show significant differences (P > 0.05) between experimental groups. The fish reared at high stocking density showed significantly lower osmolality and globulin values but higher albumin level. The HD group had significantly higher activities of catalase, glutathione peroxidase and superoxide dismutase, and malondialdehyde content in the liver when compared to the LD group. Comparative proteomics was used to determine the proteomic responses in livers of rainbow trout reared at high stocking density for 60 days. Out of nine protein spots showing altered abundance (>1.5-folds, P < 0.05), eight spots were successfully identified. Two proteins including apolipoprotein A-I-2 precursor and mitochondrial stress-70 protein were found to increase in HD group. The spots found to decrease in the HD group were identified as follows: 2-peptidylprolyl isomerase A, two isoforms of glyceraldehydes-3-phosphate dehydrogenase, an unnamed protein product similar to fructose-bisphosphate aldolase, 78 kDa glucose-regulated protein, and serum albumin 1 protein.

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Acknowledgements

We would like to thank Khorramshahr University of Marine Science and Technology for supporting this work under research grant contract no. 145.

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Authors and Affiliations

  1. Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran

    Mahdi Naderi, Saeed Keyvanshokooh & Amir Parviz Salati

  2. Agricultural Research, Education and Extension Organization, Iranian Fisheries Science Research Institute, Tehran, Iran

    Alireza Ghaedi

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  1. Mahdi Naderi
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  2. Saeed Keyvanshokooh
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Correspondence to Saeed Keyvanshokooh.

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Naderi, M., Keyvanshokooh, S., Salati, A.P. et al. Effects of chronic high stocking density on liver proteome of rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 43, 1373–1385 (2017). https://doi.org/10.1007/s10695-017-0378-8

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