Gifu University has implemented a no-smoking policy since the last ten years. Since the annual smoking rate of students significantly increased in 1998, several measures have been taken to curb this rate. Open lectures on enlightenment were initiated. Gifu University Hospital prohibited smoking in all its buildings, and Gifu University established a working group for the promotion of the no-smoking policy in 2003. In addition, enlightenment books were provided to all the students and the faculty, and the president declared a no-smoking action in 2003. In April 2005, a total smoking ban was established in all the university-owned buildings and grounds. At the same time, an anti-smoking education program for freshmen, a quitting program for smokers, and a commendation for previous smokers who successfully quit smoking were also introduced. Owing to the abovementioned measures, the total smoking rate decreased from 15.8％ in 1999 to 4.4％ in 2007 in Gifu University. The smoking rates in men and women who entered the university in 1998 increased from 9.4％ and 1.2％ in 1998 to 32.2％ and 6.4％ in 2001, respectively, in the four years of under graduation. On the other hand, the smoking rates in men and women who entered the university in 2004 increased from 3.8％ and 0.0％ in 2004 to 13.3％ and 1.1％ in 2007, respectively. Such anti-smoking activities may accelerate the decrease in students’ smoking rate. We report here as an example that the anti-smoking actions taken by the university had a positive effect on the health promotion of university students.