REARRANGEMENT OF IMMUNOGLOBULIN κ GENE IN A VARIANT-TYPE BURKITT LYMPHOMA CELL LINE, KOBK101, CARRYING A TRANSLOCATION BETWEEN CHROMOSOMES 2 AND 8

Masami FUJINO; Nobuyuki TAKAYAMA; Shingo KATO; Koichi MATSUO; Toshiya TAKANO; Shinpei NAKAZAWA

doi:10.20772/cancersci1985.77.1_24

Masami FUJINO, Nobuyuki TAKAYAMA, Shingo KATO, Koichi MATSUO, Toshiya TAKANO, Shinpei NAKAZAWA

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Keywords: C_κ region, V-J recombination, Southern blot analysis, Nucleotide sequences, c-myc

JOURNAL FREE ACCESS

1986 Volume 77 Issue 1 Pages 24-27

DOI https://doi.org/10.20772/cancersci1985.77.1_24

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Abstract

The immunoglobulin κ light-chain gene was rearranged in a Burkitt lymphoma cell line with the t(2;8) (pll;q24) translocation, KOBK101, showing recombination between the variable and the joining regions on one allele and an aberrant substitution of the region upstream of the J5 segment with an unknown sequence on another allele. Because DNA segments homologous to the consensus sequences of joining and switching regions of immunoglobulin genes were detected in the unknown sequence, we speculate that the system for either V-J or switching recombination in immunoglobulin genes may be involved in forming this unusual rearrangement.

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