Evaluation of the Chelating Efficacy of Aquilaria malaccensis and Aristolochia longa Against Biochemical Alterations Induced by Lead Bioaccumulation in Rats

Abstract

The current study was evaluated the chelating effect of Aquilaria malaccensis and Aristolochia longa against lead induced biochemical alterations and bioaccumulation of lead in tissues of rats. 25 adult female Wistar albino rats, equally divided into control and four treated groups, received either lead, lead + A. malaccensis, lead + A. longa and lead + A. malaccensis + A. longa  lead (100 mg/kg b.w) as Pb(C₂H₃O₂)₂ added in their drinking water for 75 days. A. malaccensis and A. longa (at a dose 1% of diet) were added to the feed during the last 15 days of lead exposed in the animals. Result showed that in lead-intoxicated rats, an increase of lead accumulation in serum, bone and liver of rats. Results also revealed that lead affected metabolic system by increasing blood glucose and serum urea concentrations and decreasing serum calcium concentration. Treatment with A. malaccensis and A. longa alone or combined significantly reduced the adverse effects related to most of serum and tissue lead accumulation and restored biochemical parameters alterations in animals treated with lead. The present study shows that A. malaccensis and A. longa are an effective chelating agents for the removal of lead and it has proved efficient in restoring both the biochemical alteration and toxicity after a sub lethal exposure of lead acetate in rats.