ANTIOXIDANT POTENTIAL OF TEMULAWAK (CURCUMA XANTHORRHIZA) EXTRACT GEL AS A CANDIDATE FOR WOUND HEALING
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Background: The complete treatment of chronic wounds remains a significant unmet medical need. To expedite the healing of chronic wounds, numerous studies have begun to utilize gels, including hydrogels or sol-gels, which incorporate a combination of antioxidant properties. Curcuma xanthorrhiza is known to contain polyphenolic compounds, which include flavonoids acting as an antioxidant. Purpose: Examine the potential of C. xanthorrhiza extract (CXE) gel as a candidate for wound-healing by measuring its antioxidant activity. Method: Three CXE gel formulas were prepared from different concentrations of CMC-Na (3, 4, and 5%). Each formula consisted of 5% CXE, 15% propylene Glycol, 10% Glycerin, 0.25% Methylparaben, and distilled water. The quality of the CXE gel was tested through homogeneity, spreadability, pH, and viscosity tests. The antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazil (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)-reducing activity assay in a gel formula that was close the standards. The measurement results were then used in the calculation of antioxidant activity to determine the IC50 value. Result: Formulas 1, 2, and 3 meet the requirements by yielding a homogeneous gel with a spreadability of 5.37 – 5.93 cm, a pH level of 5.87 – 6.10, and a viscosity of 8.800 – 9296.67 cps. The highest DPPH and ABTS percentages were 34.04% and 5.28%, respectively. The IC50 values of CXE gel in DPPH and ABTS were 1973.38 μg/mL and 700.65 μg/mL, respectively. Conclusion: The CXE 1, 2, and 3 gel formula meets the requirements and has the potential to be used as a wound healing therapy through its antioxidant properties.
Afianti, H. P., Murrukmihadi, M. (2015). Pengaruh Variasi Kadar Gelling Agent HPMC terhadap Sifat Fisik dan Aktivitas Antibakteri Sediaan Gel Ekstrak Etanolik Daun Kemangi (Ocimum basilicum L. forma citratum Back.). Majalah Farmaseutik, Vol. 11(2), Pp. 307-315.
Ahmad, S. U., Aladdin, N.-A. B., Jamal, J. A., Shuid, A. N., Mohamed, I. N. (2021). Evaluation of Wound-Healing and Antioxidant Effects of Marantodes pumilum (Blume) Kuntze in an Excision Wound model. Molecules, Vol. 26(1), Pp. 28.
Anggun, B. D., Slamet, S., Pambudi, D. B. (2020). Uji Stabilitas Fisik Formula Sediaan Gel Ekstrak Daun Kelor (Moringa Oleifera Lamk.). Jurnal Ilmiah Kesehatan, Vol. 13(2), Pp. 115-122.
Christodouleas, D. C., Fotakis, C., Nikokavoura, A., Papadopoulos, K., Calokerinos, A. C. (2015). Modified DPPH and ABTS Assays to Assess The Antioxidant Profile of Untreated Oils. Food Analytical Methods, Vol. 8(5), Pp.1294–1302.
Comino-Sanz, I. M., Lopez-Franco, M. D., Castro, B., Pancorbo-Hidalgo, P. L. (2021). The Role of Antioxidants on Wound Healing: A Review of The Current Evidence. Journal of Clinical Medical, Vol.10(16), Pp. 3558.
Deng, Z., Shi, F., Zhou, Z., Sun, F., Sun, M.-H., Sun, Q., Chen, L., Li, D., Jiang, C.-Y., Zhao, R.-Z., Cui, D., Wang, X.-J., Jing, Y.-F., Xia, S.-J., Han, B.-M. (2019). M1 Macrophage Mediated Increased Reactive Oxygen Species (ROS) Influence Wound Healing via The MAPK Signaling in Vitro and in Vivo. Toxicol Appl Pharmacology, Vol. 366, Pp.83-95.
Devaraj, S., Ismail, S., Ramanathan, S., Yam, M. F. (2014). Investigation of Antioxidant and Hepatoprotective Activity of Standardized Curcuma xanthorrhiza Rhizome in Carbon Tetrachloride-Induced Hepatic Damaged Rats. Scientific World Journal, Pp. 353128.
Dosoky, N. S., Setzer, W. N. (2018). Chemical Composition and Biological Activities of Essential Oils of Curcuma Species. Nutrients, Vol. 10(9), Pp. 1196.
Fuchs, S., Shariati, K., Ma, M. (2020). Specialty Tough Hydrogels and Their Biomedical Applications. Advanced Healthcare Materials, Vol. 9(2), Pp. e1901396.
Gharibi, R., Yeganeh, H., Rezapour-Lactoee, A., Hassan, Z.M. (2015). Stimulation of Wound Healing byElectroactive, Antibacterial, and AntioxidantPolyurethane/Siloxane Dressing Membranes: inVitro and in Vivo Evaluations. ACS Applied Materials & Interfaces, Vol. 7(43), Pp. 24296-24311.
Ghomi, E. R., Khalili, S., Khorasani, S. N., Neisiany, R. E., Ramakrishna, S. (2019). Wound Dressings: Current Advances and Future Directions. Journal of Applied Polymer Science, Vol. 136(27), Pp. 47738.
Ginting, C. N., Lister, I. N. E., Girsang, E., Riastawati, D. (2020). Antioxidant Activities of Ficus Elastica Leaves Ethanol Extract and Its Compounds. Molecular and Cellular Biomedical Sciences, Vol. 4(1), Pp. 27-33.
Gunes, S., Tamburaci, S., Dalay, M. C., Gurhan, I. D. (2017). In Vitro Evaluation of Spirulina Platensis Extract Incorporated Skin Cream with Its Wound Healing and Antioxidant Activities. Pharmaceutical Biology, Vol. 55(1), Pp. 1824-1832.
Hadisi, Z., Nourmohammadi, J., Nassiri, S. M. (2018). The Antibacterial and Anti-Inflammatory Investigation of Lawsonia Inermis-Gelatin-Starch Nano-Fibrous Dressing in Burn Wound. International Journal of Biological Macromolecules, Vol.107(Part B), Pp. 2008-2019.
Hakkarainen, T., Koivuniemi, R., Kosonen, M., Escobedo-Lucea, C., Sanz-Garcia, A., Vuola, J., Valtonen, J., Tammela, P., Mäkitie, A., Luukko, K., Yliperttula, M., Kavola, H. (2016). Nanofibrillar Cellulose Wound Dressing in Skin Graft Donor Site Treatment. Journal of Controlled Release, Vol.244(Part B), Pp. 292-301.
Hasanah, U., Yusriadi, Y., Khumaidi, A. (2017). Formulasi Gel Ekstrak Etanol Daun Kelor (Moringa oleifera Lam) sebagai Antioksidan. Online Journal of Natural Science, Vol..6(1), Pp. 46-57
Helary, C., Abed, A., Mosser, G., Louedec, L., Letourneur, D., Coradin, T., Giraud-Guille, M. M., Meddahi-Pelle, A. (2015). Evaluation of Dense Collagen Matrices asMedicated Wound Dressing for The Treatment ofCutaneous Chronic Wounds. Biomaterials Science, Vol. 3(2), Pp. 373-382.
Kesumayadi, I., Almas, A. I., Rambe, Hakim, I. N., Rebriarina, H. (2021). Effect of Curcuma xanthorrhiza Gel on Methicillin-Resistant Staphylococcus aureus-Infected Second-Degree Burn Wound in Rats. Natural Product Sciences, Vol. 27(1), Pp. 1-9.
Khan, A. W., Kotta, S., Ansar, S. H., Sharma, R. K., Kumar, A., Ali, J. (2013). Formulation Development, Optimization and Evaluation of Aloe Vera Gel for Wound Healing. Pharmacognosy Magazine, Vol. 9((Suppl. 1), Pp. S6-S10.
Koo, M.-A., Hong, S. H., Lee, M. H., Kwon, B.-J., Seon, G. M., Kim, M. S., Kim, D., Nam, K. C., Park, J.-C. (2019). Effective Stacking and Transplantation of Stem Cell Sheets using Exogenous ROS-Producing Film for Accelerated Wound Healing. Acta Biomaterialia, Vol. 95, Pp. 418-426.
Kumar, S., Sandhir, R., Ojha, S. (2014). Evaluation of Antioxidant Activity and Total Phenol in Different Varieties of Lantana camara Leaves. BMC Research Notes, Vol. 7(1), Pp. 1-9.
Kurahashi, T., Fujii, J. (2015). Roles of Antioxidative Enzymes in Wound Healing. Journal of Developmental Biology, Vol. 3(2), Pp. 57-70.
Lee, Y.-H., Chang, J.-J., Chien, C.-T., Yang, M.-C., Chien, H.-F. (2012). Antioxidant Sol-Gel Improves Cutaneous Wound Healing in Streptozotocin-Induced DiabeticRats. Experimental Diabetes Research, Pp. 504693.
Malone-Povolny, M. J., Maloney, S. E., Schoenfisch, M. H. (2019). Nitric Oxide Therapy for Diabetic WoundHealing. Advanced Healthcare Materials, Vol. 8(12), Pp. e1801210.
Mansouri, F. El, Farissi, H. El, Fadoua, A., Gueriri, S. A. El. (2021). Investigation of The Pharmacological Properties and Antioxidant Activity of Curcuma longa. L. AIP Conference Proceedings, Vol.2417(1), Pp. 20025.
Mary, H. P., Susheela, G. K., Jayasree, S., Nizzy, A., Rajagopal, B., Jeeva, S. (2012). Phytochemical Characterization and Antimicrobial Activity of Curcuma xanthorrhiza Roxb. Asian Pacific Journal of Tropical Biomedicine, Vol.2(Suppl.2), Pp. S637-S640.
Mayet, N., Yahya E Choonara, P. K., Tomar, L. K., Tyagi, C., Toit, L. C. Du, Pillay, V. (2014). A Comprehensive Review of Advanced Biopolymeric Wound Healing Systems. Journal of Pharmaceutical Sciences, Vol.103(8), Pp. 2211-2230.
Mulholland, E., Dunne, N. J., McCarthy, H. O. (2017). MicroRNA as Therapeutic Targets for Chronic Wound Healing. Molecular Therapy - Nucleic Acids, Vol. 8(C), Pp. 1-21.
Nguyen, T. T. T., Ghosh, C., Hwang, S.-G., Tran, L. D., Park, J. S. (2013). Characteristics of Curcumin-Loaded Poly (Lactic Acid) Nanofibers for Wound Healing. Journal of Materials Science, Vol. 48, Pp. 7125–7133.
O’Connor, N. A., Syed, A., Wong, M., Hicks, J., Nunez, G., Jitianu, A., Siler, Z., Peterson, M. (2020). Polydopamine Antioxidant Hydrogels for Wound Healing Applications. Gels, Vol. 6(4), Pp. 39.
Rahmat, E., Lee, J., Kang, Y. (2021). Javanese turmeric (Curcuma xanthorrhiza Roxb.): Ethnobotany, Phytochemistry, Biotechnology, and Pharmacological Activities. Evidence-Based Complementary and Alternative Medicine, Vol. 11.
Ramdani, E. D., Marlupi, U. D., Sinambela, J. M., Tjandrawinata, R. (2016). A New Method of Xanthorrhizol Isolation from The Rhizome Extract of Curcuma xanthorrhiza. Scholars Academic Journal of Biosciences, Vol. 4, Pp.732-737.
Rohman, A., Widodo, H., Lukitaningsih, E., Rafi, M. (2019). Review on in Vitro Antioxidant Activities of Curcuma Species Commonly Used as Herbal Components in Indonesia. Food Research, Vol. 4(2), Pp. 286-293.
Rohman, A., Wijayanti, T., Windarsih, A., Riyanto, S. (2020). The Authentication of Java Turmeric (Curcuma xanthorrhiza) using Thin Layer Chromatography and 1H-NMR based-Metabolite Fingerprinting Coupled with Multivariate Analysis. Molecules, Vol. 25(17).
Rosidi, A. (2020). The Difference of Curcumin and Antioxidant Activity in Curcuma xanthorrhiza at Different Regions. Journal of Advanced Pharmacy Education & Research, Vol. 10(1), Pp. 14-18.
Rosidi, A., Khomsan, A., Setiawan, B., Riyadi, H. (2016). Antioxidant Potential of Temulawak (Curcuma xanthorrhiza roxb). Pakistan Journal of Nutrition, Vol. 15(6), Pp. 556-560.
Sahoo, A., Jena, S., Ray, A., Dash, K. T., Nayak, S., Panda, P.C. (2021). Chemical Constituent Analysis andAntioxidant Activity of Leaf Essential Oil of Curcuma xanthorrhiza. Journal of Essential Oil Bearing Plants,Vol. 24(4).
Sayuti, N. A. (2015). Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Daun Ketepeng Cina (Cassia alata l.). Jurnal Kefarmasian Indonesia, Vol. 5(2), Pp. 74-82.
Senja, R. Y., Amelia, R. (2018). Formulasi dan Uji Stabilitas Gel Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) dengan Pembentuk Gel Carbopol 940 Konsentrasi 0.5% & 0.75% dan HPMC. Medimuh: Jurnal Kesehatan Muhammadiyah, Vol. 1(1), Pp. 45-54.
Shi, L., Liu, X., Wang, W., Jiang, L., Wang, S. (2018). A Self‐Pumping Dressing for Draining Excessive Biofluid around Wounds. Advanced Materials, Vol 31(5).
Sohn, D. H., Kim, Y. C., Oh, S. H., Park, E. J., Li, X., Lee, B. H. (2003). Hepatoprotective and Free Radical Scavenging Effects of Nelumbo Nucifera. Phytomedicine, Vol. 10(2–3), Pp. 165-169.
Sukweenadhi, J., Yunita, O., Setiawan, F., Kartini, K., Siagian, M. T., Danduru, A. P., Avanti, C. (2020). Antioxidant Activity Screening of Seven Indonesian Herbal Extract. Biodiversitas, Vol. 21(5), Pp. 2062-2067.
Süntar, I., Akkol, E. K., Nahar, L., Sarker, S. D. (2012). Wound Healing and Antioxidant Properties: Do They Coexist in Plants? Free Radicals and Antioxidants, Vol. 2(2), Pp. 1-7.
Thangavel, P., Ramachandran, B., Kannan, R., Muthuvijayan, V. (2017). Biomimetic Hydrogel Loaded with Silk and l‐Proline for Tissue Engineering and Wound Healing Applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 105(6), Pp. 1401-1408.
Tonin, L. T. D., Oliveira, T. F. V. de, Marco, I. G. de, Palioto, G.F., Düsman, E. (2021). Bioactive Compoundsand Antioxidant, Antimicrobial and CytotoxicActivities of Extracts of Curcuma longa. Journal ofFood Measurement and Characterization, Vol. 15, Pp. 3752–3760.
Widowati, W., Herlina, T., Ratnawati, H., Constantia, G., Deva, Sathya, I. D. G., Maesaroh, M. (2015a). Antioxidant Potential of Black, Green and Oolong Tea Methanol Extracts. Biology, Medicine, & Natural Product Chemistry, Vol. 4(2), Pp. 35-39.
Widowati, W., Janeva, B. W., Nadya, S., Amalia, A., Arumwardana, S., Kusuma, H. S. W., Arinta, Y. (2018). Antioxidant and Antiaging Activities of Jasminum Sambac Extract, and Its Compounds. Journal of Reports in Pharmaceutical Sciences, Vol. 7(3), Pp. 270-285.
Widowati, W., Pryandoko, D., Wahyuni, C. D., Marthania, M., Kusuma, H. S. W.,Handayani, T. (2021a). Antioxidant Properties of Soybean (Glycine max L.) Extract and Isoflavone. 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce), Pp. 1–6.
Widowati, W., Ratnawati, H., Husin, W., Maesaroh, M. (2015b). Antioxidant Properties of Spice Extracts. Biomedical Engineering, Vol. 1(1), Pp. 24-29.
Widowati, W., Tjokropranoto, R., Damayanti, C., Kusuma, H., Handayani, T., Rizal, R. (2021b). Potential of Black Tea (Camellia Sinensis (L.) O. Kuntze) Extract as Anti-oxidant and Skin Anti-aging.
Widyastuti, I., Luthfah, H. Z., Hartono, Y. I., Islamadina, R., Can, A. T., Rohman, A. (2021). Antioxidant Activity of Temulawak (Curcuma xanthorrhiza Roxb.) and Its Classification with Chemometrics. Indonesian Journal of Chemometrics and Pharmaceutical Analysis, Vol. 1(1), Pp. 28-41.
Xu, Z., Han, S., Gu, Z., Wu, J. (2020). Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing. Advanced Healthcare Materials, Vol. 9(5), Pp.1901502.
Zhang, C.-M., Fan, P.-H., Li, M., Lou, H.-X. (2014). Two New Sesquiterpenoids from The Rhizomes of Curcuma xanthorrhiza. Helvetica Chimica Acta, Vol. 97(9), Pp. 5-1300.
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