Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity
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Background: White spot lesion is a demineralization process indicated by the increased of enamel microporosity. A tegillarca granosa shell contains 98.7% calcium and Vitis vinifera contains phytochemical compounds with fluoride, which has a potential to stimulate remineralization. Purpose: To analyze the Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity. Methods: The cream was prepared by combining 10% and 20% Tegillarca granosa shell with 10 grams of Vitis vinifera extract and 100 mg of fluoride. The cream was tested beforehand for viscocity and pH. Furthermore, 16 premolars were etched and divided into four groups. Group 1 was smeared with placebo (negative control) and Group 2 was smeared with casein phosphopeptide-amorphous calcium phosphate (positive control). The other groups were smeared with cream 10% (Group 3) and 20% (Group 4) Tegillarca granosa shell combination with Vitis vinifera and fluoride. Teeth were treated three times a day for 30 minutes and soaked in artificial saliva. After 14 days, the enamel microporosity was carried out using a scanning electron microscope. The data was analyzed with one-way analysis of variance (ANOVA) test followed by post-hoc least significant difference (LSD). Results: The enamel microporosity showed significant difference between Group 1 and the other groups. There was no significant difference between Groups 2, 3, and 4 (p<0.05). Although there was no significant difference between Group 3 and 4, the lowest one was in Group 4 (p>0.05). Conclusion: The cream, prepared by combining Tegillarca granosa shell with Vitis vinifera and fluoride, is effective in decreasing the enamel microporosity.
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Haikal M, Adhani R, Wardani I. Hubungan laju aliran saliva terhadap kejadian karies gigi pada penderita hipertensi yang mengonsumsi obat antihipertensi. Dentin J Kedokt Gigi. 2020; 4(2): 39–42. web: http://ppjp.ulm.ac.id/journals/index.php/dnt/article/view/2283
Kunarti S, Saraswati W, Lashari DM, Salma N, Nafatila T. Enamel remineralisation-inducing materials for caries prevention. Dent J. 2021; 54(3): 165–8. doi: https://doi.org/10.20473/j.djmkg.v54.i3.p165-168
Triwardhani A, Budipramana M, Sjamsudin J. Effect of different white-spot lesion treatment on orthodontic shear strength and enamel morphology: In vitro study. J Int Oral Heal. 2020; 12(2): 120–8. doi: https://doi.org/10.4103/jioh.jioh_206_19
Arifa MK, Ephraim R, Rajamani T. Recent advances in dental hard tissue remineralization: A review of literature. Int J Clin Pediatr Dent. 2019; 12(2): 139–44. doi: https://doi.org/10.5005/jp-journals-10005-1603
Carabelly AN, Puspitasari D, Syahrina F, Wahyudi MD, Salsabila Karno DA, Mohd-Said S. Demineralized dentin characteristics after application of Mauli banana stem gel. Dent J. 2024; 57(1): 33–7. doi: https://doi.org/10.20473/j.djmkg.v57.i1.p33-37
Metly A, Sumantri D, Oenzil F. The effect of pasteurized milk and pure soy milk on enamel remineralization. Padjadjaran J Dent. 2019; 31(3): 202. doi: https://doi.org/10.24198/pjd.vol31no3.22833
Rahayu YC. Peran agen remineralisasi pada lesi karies dini. Stomatognatic (JKG Unej). 2013; 10(1): 25–30. web: https://jurnal.unej.ac.id/index.php/STOMA/article/view/2019
Dianti F, Triaminingsih S, Irawan B. Effects of miswak and nano calcium carbonate toothpastes on the hardness of demineralized human tooth surfaces. J Phys Conf Ser. 2018; 1073(3): 032008. doi: https://doi.org/10.1088/1742-6596/1073/3/032008
Adiba SH, Effendy R, Zubaidah N. Fluoride varnish effect on dental erosion immersed with carbonated beverages. J Int Dent Med Res. 2018; 11(1): 299–302. web: http://www.jidmr.com/journal/wp-content/uploads/2018/04/57D17_463_Ruslan_Effendy_Alexander_Nugraha8.pdf
Abdelnabi A, Hamza NK, El-Borady OM, Hamdy TM. Effect of different formulations and application methods of coral calcium on its remineralization ability on carious enamel. Open Access Maced J Med Sci. 2020; 8(D): 94–9. doi: https://doi.org/10.3889/OAMJMS.2020.4689
Ritter A V, Boushell LW, Walter R. Sturdevant’s art and science of operative dentistry. 2019. p. 417–18. web: https://linkinghub.elsevier.com/retrieve/pii/C20150056039
Apriani A, Naliani S, Djuanda R, Teanindar SH, Florenthe JQ, Baharudin F. Surface roughness assessment with fluoride varnish application: An in vitro study. Dent J. 2023; 56(3): 154–9. doi: https://doi.org/10.20473/j.djmkg.v56.i3.p154-159
Rachmawati D, Kurniawati C, Hakim L, Roeswahjuni N. Efek remineralisasi casein phospopeptide-amorphous calcium phospate (CPP-ACP) terhadap enamel gigi sulung. E-Prodenta J Dent. 2019; 3(2): 257–62. web: https://eprodenta.ub.ac.id/index.php/eprodenta/article/view/59
Prananingrum W, Prabowo PB. The increasing of enamel calcium level after casein phosphopeptideamorphous calcium phosphate covering. Dent J. 2012; 45(2): 93–6. doi: https://doi.org/10.20473/j.djmkg.v45.i2.p93-96
Yuanita T, Zubaidah N, A R MI. Enamel hardness differences after topical application of theobromine gel and casein phosphopeptide-amorphous calcium phosphate. Conserv Dent J. 2020; 10(1): 5–8. doi: https://doi.org/10.20473/cdj.v10i1.2020.5-8
Matsui T, Naito M, Kitamura K, Makino A, Sugiura S, Izumi H, Ito K. Casein phosphopeptide in cow ’ s milk is strongly allergenic. Authorea. 2021; : 1–4. doi: https://doi.org/10.22541/au.163252091.16716154/v1
Flom JD, Sicherer SH. Epidemiology of cow’s milk allergy. Nutrients. 2019; 11(5): 1051. doi: https://doi.org/10.3390/nu11051051
Moonesinghe H, Mackenzie H, Venter C, Kilburn S, Turner P, Weir K, Dean T. Prevalence of fish and shellfish allergy. Ann Allergy, Asthma Immunol. 2016; 117(3): 264-272.e4. doi: https://doi.org/10.1016/j.anai.2016.07.015
Sawiji A, Perdanawati RA. Pemetaan pemanfaatan limbah kerang dengan pendekatan masyarakat berbasis aset (Studi kasus: Desa Nambangan Cumpat, Surabaya). Mar J. 2017; 03(01): 10–9. web: https://jurnalsaintek.uinsby.ac.id/mhs/index.php/marine/article/view/42
Ma’ruf A, Hartati S. Production and characterization of nano-chitosan from blood clamshell (Anadara granosa) by ionic gelation. Nat Environ Pollut Technol. 2022; 21(4): 1761–6. doi: https://doi.org/10.46488/NEPT.2022.v21i04.031
Nastiti AD, Widyastuti W, Laihad FM. Bioviabilitas hidroksiapatit ekstrak cangkang kerang darah (Anadara granosa) terhadap sel punca mesenkimal sebagai bahan graft Tulang. Dent J Kedokt Gigi. 2015; 9(2): 122–8. web: https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/161
Sari RP, Sudjarwo SA, Rahayu RP, Prananingrum W, Revianti S, Kurniawan H, Bachmid AF. The effects of Anadara granosa shell-Stichopus hermanni on bFGF expressions and blood vessel counts in the bone defect healing process of Wistar rats. Dent J. 2017; 50(4): 194–8. doi: https://doi.org/10.20473/j.djmkg.v50.i4.p194-198
Lopata AL, Kleine-Tebbe J, Kamath SD. Allergens and molecular diagnostics of shellfish allergy. Allergo J Int. 2016; 25(7): 210–8. doi: https://doi.org/10.1007/s40629-016-0124-2
Zailatul HMY, Rosmilah M, Faizal B, Noormalin A, Shahnaz M. Malaysian cockle (Anadara granosa) allergy: Identification of IgE-binding proteins and effects of different cooking methods. Trop Biomed. 2015; 32(2): 323–34. pubmed: http://www.ncbi.nlm.nih.gov/pubmed/26691261
Busman B, Edrizal E, Utami DWP. Uji efektivitas ekstrak buah anggur hijau (Vitis Vinivera L) terhadap daya hambat laju pertumbuhan bakteri Streptococcus mutans dan Lactobacillus acidophilus. Ensiklopedia Sos Rev. 2021; 2(3): 325–32. doi: https://doi.org/10.33559/esr.v2i3.623
Jawale KD, Kamat SB, Patil JA, Nanjannawar GS, Chopade RV. Grape seed extract: An innovation in remineralization. J Conserv Dent. 2017; 20(6): 415–8. doi: https://doi.org/10.4103/JCD.JCD_287_16
Ahmad I. Pemanfaatan limbah cangkang kerang darah (Anadara granosa) sebagai bahan abrasif dalam pasta gigi. J Galung Trop. 2017; 6: 49–59. doi: https://doi.org/10.31850/JGT.V6I1.210
Maulana F, Mulawarmanti D, Laihad FM. Combination administration of Stichopus hermanii gel and hyperbaric oxygen therapy the number of fibroblasts in diabetes mellitus rats with periodontitis. Dent J Kedokt Gigi. 2017; 11(2): 9–17. web: https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/87
Makmur SA, Utomo RB. Pengaruh aplikasi gel Theobromine terhadap kekasaran permukaan email gigi desidui pasca demineralisasi. ODONTO Dent J. 2019; 6(2): 95–8. doi: https://doi.org/10.30659/odj.6.2.95-98
Ari DPS, Yonatasya FD, Saftiarini G, Prananingrum W. Variations of gelatin percentages in HA-TCP scaffolds as the result of 6- and 12-hour sintering processes of blood cockle (Anadara granosa) shells against porosity. Dent J. 2018; 51(4): 158–63. doi: https://doi.org/10.20473/j.djmkg.v51.i4.p158-163
Pamungkas GB, Karunia D, Suparwitri S. Desensitizing agents’ post-bleaching effect on orthodontic bracket bond strength. Dent J. 2024; 57(1): 45–9. doi: https://doi.org/10.20473/j.djmkg.v57.i1.p45-49
Wulandari E, Wardani FRA, Fatimattuzahro N, Dewanti IDAR. Addition of gourami (Osphronemus goramy) fish scale powder on porosity of glass ionomer cement. Dent J. 2022; 55(1): 33–7. doi: https://doi.org/10.20473/j.djmkg.v55.i1.p33-37
Mailana D, Nuryanti, Harwoko. Formulasi sediaan krim antioksidan ekstrak etanolik daun alpukat (Persea americana Mill.). Acta J Indones. 2016; 4(2): 7–15. web: http://jos.unsoed.ac.id/index.php/api/article/view/1468
Nurjannah W, Yusriadi, Nugrahani AW. Uji aktivitas antibakteri formula pasta gigi ekstrak batang karui (Harrisonia Perforata Merr.) terhadap bakteri Streptococcus mutans. Biocelebes. 2018; 12(2): 52–61. web: https://bestjournal.untad.ac.id/index.php/Biocelebes/article/view/10747
Dianawati N, Setyarini W, Widjiastuti I, Ridwan RD, Kuntaman K. The distribution of Streptococcus mutans and Streptococcus sobrinus in children with dental caries severity level. Dent J. 2020; 53(1): 36–9. doi: https://doi.org/10.20473/j.djmkg.v53.i1.p36-39
Hediana VAK, Probosari N, Setyorini D. Lama perendaman gigi di dalam air perasan jeruk nipis (Citrus aurantifolia Swingle) mempengaruhi kedalaman porositas mikro email (Duration of immersing teeth in lime (Citrus aurantifolia Swingle) juice affects on microporosity depth of enamel). J Dentomaxillofacial Sci. 2015; 14(1): 45–9. doi: https://doi.org/10.15562/jdmfs.v14i1.425
Junaidi, Sinala S. Farmasi fisik: Bahan ajar farmasi. Jakarta: Kementerian Kesehatan Republik Indonesia; 2017. p. 1–51. web: https://perpus.poltekkesjkt2.ac.id/respoy/index.php?p=show_detail&id=444&keywords=
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