In this paper, we present the result of clove bud oil extraction with supercritical CO2, GC/MS analysis and a genotoxicity evaluation by comet assay of its components. Clove bud oil could be extracted with supercritical CO2 efficiently. The extracts contained eugenol and various terpenes as the major constituents, similar to the commercially available clove bud oil. By adding the clove bud oil to natural cells, DNA damages caused by ultraviolet irradiation were quickly recovered. The viability of the cells increased in comparison with that of control cells. These results suggest that the clove bud oil effectively prevents the carcinogenesis caused by DNA damages.
