Efectos de un extracto de hojas de Leucaena leucocephala en la actividad de xantina oxidasa y en los niveles séricos de oxipurinas en ratones
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https://doi.org/10.18633/biotecnia.v26.2155Palabras clave:
alopurinol, teoría de Chou-Talalay, sinergismo, hipoxantinaResumen
Hay una necesidad de nuevas alternativas al uso medicinal de alopurinol. En este sentido, el presente estudio obtuvo un extracto de hojas de L. leucocephala al cual se le determinó su composición química, acción inhibitoria contra xantina oxidasa (XO) in vitro, interacción inhibitoria entre el extracto y alopurinol, y acción inhibitoria contra XO in vivo empleando ratones bajo administración de hipoxantina y oxonato de potasio. Compuestos polifenoles y flavonoides fueron detectados y cuantificados en el extracto de hojas. Para el extracto solo, su valor IC50 y máximo de inhibición contra XO fueron de 334.60 µg/mL y 46.4 %. La combinación a una proporción 3:1 de alopurinol y extracto obtuvo valores IC50 y waDRI de 1.35 µg/mL, 1.13 (alopurinol) y 1015.72 (extracto) para inhibir a XO, resultando en una interacción sinérgica contra XO in vitro. Donde esta misma combinación incrementó el éxito terapéutico en el modelo murino al compararse con la administración de alopurinol solo. Nuestro estudio presenta la primera evidencia para el uso de una combinación de alopurinol y extracto de L. leucocephala a una proporción 3:1 como un sustituto para la administración de alopurinol solo.
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