Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury
Abstract
Purpose: In this study, the effect of applying different doses of Ficus carica (fig) seed oil obtained by cold pressing method on the kidney tissue and serum GSH level, as well as the formation of necrosis and cast in the experimental myoglobinuric acute kidney injury animal model created with glycerol was investigated.
Materials and Methods: 32 Wistar albino male rats weighing 460-540 g were randomly divided into four groups of 8 each. Sham Control, MAKI, MAKI+FC3, MAKI+FC6. Urea and creatinine levels of the groups were analyzed by biochemical method. Tissue necrosis level was determined by histological analysis of kidney tissue sections.
Results: While urea and creatinine levels increased significantly in the MAKI group compared to all groups, they were found to be lower in the high and low dose treatment groups with no significant difference between them. Tissue and serum GSH levels in the MAKI group were significantly decreased compared to all groups. In the MAKI+FC3 and MAKI+FC6 groups, an increase was detected in the tissue without dose difference, and in the serum only with high dose. The highest score in kidney tissue cast and necrosis levels were observed in the MAKI group, while significant improvements were detected in the treatment groups.
Conclusion: Ficus carica(fig) seed oil, provided improvement in morphological damage with improvement in functional damage and increase in antioxidative capacity.
Keywords
Antioxidant Ficus carica seed oil myoglobinuric acute kidney injury necrosis reduced glutathione.
Supporting Institution
Aydin Adnan Menderes University, Scientific Research Projects Committee
Project Number
TPF-19054
References
- Sever L. Crush syndrome. Turk Arch Pediatrics. 2009;44:43-7.
- Slater MS, Mullins RJ. Rhabdomyolysis and myoglobinuric renal failure in trauma and surgical patients: a review. J Am Coll Surg. 1998;186:693-716.
- Lima RS, Silva Junior DS, Liborio AB, Daher ED. Acute kidney injury due to rhabdomyolysis. Saudi J Kidney Dis Transpl. 2008;19:721-9.
- Pal Singh A, Muthuraman A, Singh Jaggi A, Singh N, Grover K, Dhawan R. Animal models of acute renal failure. Pharmacol Rep. 2012;64:33-44.
- Chatzizisis YS, Misirli G, Hatzitolios AI, Giannoglou GD. The syndrome of rhabdomyolysis: complications and treatment. Eur J Intern Med. 2008;19:568-74.
- Efstratiadis G, Voulgaridou A, Nikiforou D, Kyventidis A, Kourkouni EG. Rhabdomyolysis updated. Hippokratia. 2007;11:129-37.
- Vanholder WR, Sever MS, Erek E, Lameire N. Rhabdomyolysis. J Am Soc Nephrol. 2000;11:1553-61.
- Fernández-Fúnez A, Polo FJ, Broseta L, Atienza MP, Mora A, Gascón FG. Evolution of total antioxidant status in an acute renal failure model in rats. Ren Fail. 2003;25:535-43.
- Petajova N, Martinek A. Acute kidney injury due to rhabdomyolysis and renal replacement therapy: a critical review. Crit Care. 2014;18:224.
- Boutaud O, Moore KP, Reeder BJ, Harry D, Howie A.J, Wang S et al. Acetaminophen inhibits hemoprotein-catalyzed lipid peroxidation and attenuates rhabdomyolysis-induced renal failure. Proc Natl Acad Sci U S A. 2010;107:2699-704.
- Ikeda M, Prachasilchai W, Burne-Taney MJ, Rabb H, Ikeda N. Ischemic acute tubular necrosis models and drug discovery: a focus on cellular inflammation. Drug Discov Today. 2006;11:364-70.
- Shulman LM, Yuhas Y, Frolkis I, Gavendo S, Knecht A, Eliahou HE. Glycerol induced ARF in rats is mediated by tumor necrosis factor-α. Kidney Int. 1993;43:1397-401.
- Gül Y, Özrenk K. Fig (Ficus carica L.) A study on the determination of genetic sources: Turkey, Siirt region. Turk Tarim Arast Derg. 2019;6:328-35.
- Solomon A, Golubowicz S, Yablowicz Z, Grossman S, Bergman M, Gottlieb HE et al.. Antioxidant activities and anthocyanin content of fresh fruits of common fig (Ficus carica L.). J Agric Food Chem. 2006;54:7717-23.
- Çalışkan O, Polat AA. Determination of phytochemical and antioxidant properties of some fig varieties. Journal of Agriculture Faculty of Ege University. 2012;49:201-8.
- Nakilcioğlu E, Hışıl Y. Research on the phenolic compounds in sarılop (Ficus carıca l.) fig variety. J Food. 2013;38:267-74.
- Duman E, Yazici AS. Physico-chemical properties of fresh fig (purple autumn - yellow lop) seed and seed oils. Journal of Anadolu Ege Agricultural Research Institute. 2018;28:69-76
- Joseph B, Raj SJ. Pharmacognostic and phytochemical properties of Ficus carica linn –an overview. Int J PharmTech Res. 2011;3:08-12.
- Güven N, Gökyer A, Koç A, Temiz NN, Selvi S, Koparal B. et al. Physiochemical composition of fig seed oil fromt Turkey. J Pharm Pharmacol. 2019;541-45.
- Baygeldi N, Küçükerdönmez Ö, Akder RN, Çağındı Ö. Medicinal and nutritional analysis of fig (Ficus carica) seed oil; a new gamma tocopherol and omega-3 source. Progress in Nutrition. 2021;23:e2021052
- Mostafaie A, Mansouri K, Norooznezhad A, Mohammadi-Motlagh H. Anti angiogenic activity of Ficus carica latex extract on human umbilical vein endothelial cells. Yakhteh. 2011;12:525–8.
- Gond NY, Khadabadi SS. Hepatoprotective activity of Ficus carica leaf extract on rifampicininduced hepatic damage in rats. Indian J Pharm Sci.2008;70:364-6.
- Asadi F, Pourkabir M, Maclaren R, Shahriar A. Alterations to lipid parameters in response to fig tree (Ficus carica) leaf extract in chicken liver slices. Turk J Vet Anim Sci. 2006;30:315-8.
- Chander V, Singh D, Chopra K. Catechin, a natural antioxidant protects against rhabdomyolysis induced myoglobinuric acute renal failure. Pharmacol Res. 2003;48:503-9.
- Arslan K, Hüseyin S, Yüksel V, Yalta TD, Turan FN, Canbaz S et al. Effects of papaverine and ascorbic acid on reperfusion induced renal injury: an experimental ischemia and reperfusion models. J Cardiovasc Sci. 2014;26:69-78.
- Güzel D, Tanyeli A. p-coumaric acid reduces renal ischemia reperfusion-induced acute lung injury. Sakarya Tıp Dergisi 2018;8:644-649.
- Bradford MM, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem.1976;72:248-54.
- Cardiff RD, Miller CH, Munn RJ. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc. 2014;655-8.
- Tsai JP, Lee C, Hsieh YH, Hsu BG. Calcitriol ameliorated rhabdomyolysis induced acute renal failure in rats. Int J Clin Exp Med.2017;10:2430-9.
- Al Asmari AK, Sadoon KTA, Obaid AA, Yesunayagam D, Tariq M. Protective effect of quinacrine against glycerol-induced acute kidney injury in rats. BMC Nephrol. 2017;18:41.
- Üstündağ S, Yalçın Ö, Şen S, Çukur Z, Çiftçi Ş, Demirkan B. Experimental myoglobinuric acute renal failure: the effect of vitamin c. Ren Fail. 2008;30:727-35.
- Oken DE, Dibona GF, Mcdonald FD. Micropuncture studies of the recovery phase of myohemoglobinuric acute renal failure in the rat. J Clin Invest. 1970;49:730-7.
- Al Laham SA. The curative effects methylsulfonylmethane against glycerolinduced acute renal failure in rats. Braz J Pharm Sci. 2018;54:e17442.
- Moggio A, Geraci S, Boido A, Sticht C, Gretz N, Bussolati B. Assessment of acute kidney injury in rhabdomyolytic mice by transcutaneous measurement of sinistrin excretion. Nephrol Dial Transplant. 2017;32:1167-75.
- Vlahovic, P, Cvetkovic T, Savic V, Stefanovic V. Dietary curcumin does not protect kidney in glycerol-induced acute renal failure. Food Chem Toxicol. 2007;45:1777-82.
- Fatoni AZ, Kestriani ND. Acute kidney injury (AKI) in critically ill patients. Anesth Crit Care. 2018;36:64-76.
- De Mendonca A, Vincent JL, Suter PM, Moreno R, Dearden NM, Antonelli M, et al. Acute renal failure in the ICU: risk factors and outcome evaluated by the SOFA Score. Intensive Care Med. 2000;26:915-21.
- Abul-Ezz S, Walker PD, Shah SV. The role of glutathione in an animal model of myoglobinuric acute renal failure. Proc Natl Acad Sci U S A. 1991;88:9833-7.
- Nara A, Yajima D, Nagasawa S, Abe H, Hoshioka Y, Iwase H. Evaluations of lipid peroxidation and inflammation in short‐term glycerol‐induced acute kidney injury in rats. Clin Exp Pharmacol Physiol. 2016;43:1080-86.
- Tajik J, Kheirandish R, Rohani H, Abbasi S Protective effects of vitamin E in experimental rhabdomyolysis. Eurasian J Vet Sci. 2013;29:180-4.
- Orak C, Şirinyıldız F, Yılmaz EG, Cesur G, Ek RO. Protective effects of Ficus carica seed oil on ischemia and reperfusion injury in a rat model of acute mesenteric ischemia. Ulus Travma Acil Cerrahi Derg. 2021;27:402-9.
- Şirinyıldız F. The investigation of possible effects of fig (Ficus carica) seed oil on experimental animal model colitis that induceb by TNBS (Doctoral thesis). Aydın, Aydın Adnan Menderes University, 2020..
- Taşlı Yeşilçayır G. Therapeutic effects of fig ( Ficus carica) seed oil on paracetamol induced acute hepatotoxicity (Doctoral thesis). Aydın, Aydın Adnan Menderes University, 2021.
Miyoglobinürik akut böbrek hasarında Ficus carica (incir) çekirdek yağı uygulamasının GSH düzeyleri, nekroz ve kast oluşumu üzerine etkisi
Abstract
Amaç: Bu çalışmada, Gliserolle oluşturulmuş Deneysel Miyoglobinürük akut böbrek hasarı hayvan modelinde, soğuk pres yöntemiyle elde edilmiş olan Ficus carica (incir) çekirdeği yağının farklı dozlarda uygulanmasının, böbrek dokusu ve serum GSH düzeyi, ayrıca nekroz ve kast oluşumu üzerindeki etkisi araştırılmıştır.
Gereç ve Yöntem: Ağırlığı 460-540 gr arasında değişen, 32 adet Wistar albino erkek sıçan randomize 8’erli dört gruba ayrıldı. Sham Kontrol, MAKI, MAKI+FC3, MAKI+FC6. Grupların Üre ve kreatinin düzeyleri biyokimyasal yöntemle analiz edildi. Doku nekroz düzeyi böbrek doku kesitlerinin histolojik analizi ile belirlendi.
Bulgular: MAKI grubunda üre ve kreatinin düzeyi tüm gruplara göre anlamlı olarak artış gösterirken, yüksek ve düşük doz tedavi grubunda aralarında anlamlı fark olmazsızın düşük tespit edildi. MAKI grubu doku ve serum GSH düzeyi tüm gruplara göre anlamlı olarak azaldı. MAKI+FC3 ve MAKI+FC6 gruplarında, dokuda doz farkı olmaksızın, serumda ise yalnız yüksek doz ile artış tespit edildi. Böbrek dokusu kast ve nekroz düzeyinde en yüksek skor MAKI grubunda gözlenirken, tedavi gruplarında anlamlı düzeyde iyileşme tespit edildi.
Sonuç: Ficus carica (incir) çekirdeği yağı ise, fonksiyonel hasarda iyileşme ve antioksidatif kapasitede artış ile birlikte morfolojik hasarda iyileşme sağlamıştır.
Keywords
Antioksidan Ficus carica çekirdek yağı indirgenmiş glutatyon miyoglobinürik akut böbrek hasarı nekroz.
Project Number
TPF-19054
References
- Sever L. Crush syndrome. Turk Arch Pediatrics. 2009;44:43-7.
- Slater MS, Mullins RJ. Rhabdomyolysis and myoglobinuric renal failure in trauma and surgical patients: a review. J Am Coll Surg. 1998;186:693-716.
- Lima RS, Silva Junior DS, Liborio AB, Daher ED. Acute kidney injury due to rhabdomyolysis. Saudi J Kidney Dis Transpl. 2008;19:721-9.
- Pal Singh A, Muthuraman A, Singh Jaggi A, Singh N, Grover K, Dhawan R. Animal models of acute renal failure. Pharmacol Rep. 2012;64:33-44.
- Chatzizisis YS, Misirli G, Hatzitolios AI, Giannoglou GD. The syndrome of rhabdomyolysis: complications and treatment. Eur J Intern Med. 2008;19:568-74.
- Efstratiadis G, Voulgaridou A, Nikiforou D, Kyventidis A, Kourkouni EG. Rhabdomyolysis updated. Hippokratia. 2007;11:129-37.
- Vanholder WR, Sever MS, Erek E, Lameire N. Rhabdomyolysis. J Am Soc Nephrol. 2000;11:1553-61.
- Fernández-Fúnez A, Polo FJ, Broseta L, Atienza MP, Mora A, Gascón FG. Evolution of total antioxidant status in an acute renal failure model in rats. Ren Fail. 2003;25:535-43.
- Petajova N, Martinek A. Acute kidney injury due to rhabdomyolysis and renal replacement therapy: a critical review. Crit Care. 2014;18:224.
- Boutaud O, Moore KP, Reeder BJ, Harry D, Howie A.J, Wang S et al. Acetaminophen inhibits hemoprotein-catalyzed lipid peroxidation and attenuates rhabdomyolysis-induced renal failure. Proc Natl Acad Sci U S A. 2010;107:2699-704.
- Ikeda M, Prachasilchai W, Burne-Taney MJ, Rabb H, Ikeda N. Ischemic acute tubular necrosis models and drug discovery: a focus on cellular inflammation. Drug Discov Today. 2006;11:364-70.
- Shulman LM, Yuhas Y, Frolkis I, Gavendo S, Knecht A, Eliahou HE. Glycerol induced ARF in rats is mediated by tumor necrosis factor-α. Kidney Int. 1993;43:1397-401.
- Gül Y, Özrenk K. Fig (Ficus carica L.) A study on the determination of genetic sources: Turkey, Siirt region. Turk Tarim Arast Derg. 2019;6:328-35.
- Solomon A, Golubowicz S, Yablowicz Z, Grossman S, Bergman M, Gottlieb HE et al.. Antioxidant activities and anthocyanin content of fresh fruits of common fig (Ficus carica L.). J Agric Food Chem. 2006;54:7717-23.
- Çalışkan O, Polat AA. Determination of phytochemical and antioxidant properties of some fig varieties. Journal of Agriculture Faculty of Ege University. 2012;49:201-8.
- Nakilcioğlu E, Hışıl Y. Research on the phenolic compounds in sarılop (Ficus carıca l.) fig variety. J Food. 2013;38:267-74.
- Duman E, Yazici AS. Physico-chemical properties of fresh fig (purple autumn - yellow lop) seed and seed oils. Journal of Anadolu Ege Agricultural Research Institute. 2018;28:69-76
- Joseph B, Raj SJ. Pharmacognostic and phytochemical properties of Ficus carica linn –an overview. Int J PharmTech Res. 2011;3:08-12.
- Güven N, Gökyer A, Koç A, Temiz NN, Selvi S, Koparal B. et al. Physiochemical composition of fig seed oil fromt Turkey. J Pharm Pharmacol. 2019;541-45.
- Baygeldi N, Küçükerdönmez Ö, Akder RN, Çağındı Ö. Medicinal and nutritional analysis of fig (Ficus carica) seed oil; a new gamma tocopherol and omega-3 source. Progress in Nutrition. 2021;23:e2021052
- Mostafaie A, Mansouri K, Norooznezhad A, Mohammadi-Motlagh H. Anti angiogenic activity of Ficus carica latex extract on human umbilical vein endothelial cells. Yakhteh. 2011;12:525–8.
- Gond NY, Khadabadi SS. Hepatoprotective activity of Ficus carica leaf extract on rifampicininduced hepatic damage in rats. Indian J Pharm Sci.2008;70:364-6.
- Asadi F, Pourkabir M, Maclaren R, Shahriar A. Alterations to lipid parameters in response to fig tree (Ficus carica) leaf extract in chicken liver slices. Turk J Vet Anim Sci. 2006;30:315-8.
- Chander V, Singh D, Chopra K. Catechin, a natural antioxidant protects against rhabdomyolysis induced myoglobinuric acute renal failure. Pharmacol Res. 2003;48:503-9.
- Arslan K, Hüseyin S, Yüksel V, Yalta TD, Turan FN, Canbaz S et al. Effects of papaverine and ascorbic acid on reperfusion induced renal injury: an experimental ischemia and reperfusion models. J Cardiovasc Sci. 2014;26:69-78.
- Güzel D, Tanyeli A. p-coumaric acid reduces renal ischemia reperfusion-induced acute lung injury. Sakarya Tıp Dergisi 2018;8:644-649.
- Bradford MM, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem.1976;72:248-54.
- Cardiff RD, Miller CH, Munn RJ. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc. 2014;655-8.
- Tsai JP, Lee C, Hsieh YH, Hsu BG. Calcitriol ameliorated rhabdomyolysis induced acute renal failure in rats. Int J Clin Exp Med.2017;10:2430-9.
- Al Asmari AK, Sadoon KTA, Obaid AA, Yesunayagam D, Tariq M. Protective effect of quinacrine against glycerol-induced acute kidney injury in rats. BMC Nephrol. 2017;18:41.
- Üstündağ S, Yalçın Ö, Şen S, Çukur Z, Çiftçi Ş, Demirkan B. Experimental myoglobinuric acute renal failure: the effect of vitamin c. Ren Fail. 2008;30:727-35.
- Oken DE, Dibona GF, Mcdonald FD. Micropuncture studies of the recovery phase of myohemoglobinuric acute renal failure in the rat. J Clin Invest. 1970;49:730-7.
- Al Laham SA. The curative effects methylsulfonylmethane against glycerolinduced acute renal failure in rats. Braz J Pharm Sci. 2018;54:e17442.
- Moggio A, Geraci S, Boido A, Sticht C, Gretz N, Bussolati B. Assessment of acute kidney injury in rhabdomyolytic mice by transcutaneous measurement of sinistrin excretion. Nephrol Dial Transplant. 2017;32:1167-75.
- Vlahovic, P, Cvetkovic T, Savic V, Stefanovic V. Dietary curcumin does not protect kidney in glycerol-induced acute renal failure. Food Chem Toxicol. 2007;45:1777-82.
- Fatoni AZ, Kestriani ND. Acute kidney injury (AKI) in critically ill patients. Anesth Crit Care. 2018;36:64-76.
- De Mendonca A, Vincent JL, Suter PM, Moreno R, Dearden NM, Antonelli M, et al. Acute renal failure in the ICU: risk factors and outcome evaluated by the SOFA Score. Intensive Care Med. 2000;26:915-21.
- Abul-Ezz S, Walker PD, Shah SV. The role of glutathione in an animal model of myoglobinuric acute renal failure. Proc Natl Acad Sci U S A. 1991;88:9833-7.
- Nara A, Yajima D, Nagasawa S, Abe H, Hoshioka Y, Iwase H. Evaluations of lipid peroxidation and inflammation in short‐term glycerol‐induced acute kidney injury in rats. Clin Exp Pharmacol Physiol. 2016;43:1080-86.
- Tajik J, Kheirandish R, Rohani H, Abbasi S Protective effects of vitamin E in experimental rhabdomyolysis. Eurasian J Vet Sci. 2013;29:180-4.
- Orak C, Şirinyıldız F, Yılmaz EG, Cesur G, Ek RO. Protective effects of Ficus carica seed oil on ischemia and reperfusion injury in a rat model of acute mesenteric ischemia. Ulus Travma Acil Cerrahi Derg. 2021;27:402-9.
- Şirinyıldız F. The investigation of possible effects of fig (Ficus carica) seed oil on experimental animal model colitis that induceb by TNBS (Doctoral thesis). Aydın, Aydın Adnan Menderes University, 2020..
- Taşlı Yeşilçayır G. Therapeutic effects of fig ( Ficus carica) seed oil on paracetamol induced acute hepatotoxicity (Doctoral thesis). Aydın, Aydın Adnan Menderes University, 2021.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research |
| Authors | |
| Project Number | TPF-19054 |
| Publication Date | March 31, 2022 |
| Acceptance Date | January 17, 2022 |
| Published in Issue | Year 2022 Volume: 47 Issue: 1 |
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