ISHS Acta Horticulturae 871: IV International Symposium on Cucurbits
GENETIC VARIATION ANALYSIS OF THE NEWLY SYNTHESIZED ALLOTETRAPLOIDS CUCUMIS HYTIVUS USING RETROTRANSPOSON-BASED SSAP MARKERS |
| Authors: | Biao Jiang, Qunfeng Lou, Jinfeng Chen |
| Keywords: | Cucumis, interspecific hybridization, reciprocal cross, allotetraploid, SSAP, genetic variation |
| DOI: | 10.17660/ActaHortic.2010.871.78 |
| Abstract:
Ty1-copia-like retrotransposons have been found to comprise the most common class of transposable elements in plant and occur in high copy number in plant genomes. The dispersion, ubiquity and prevalence of Ty1-copia retrotransposons in plant genomes potentially provide an excellent system for developing a multiplex DNA-based marker system. Sequence-specific amplification polymorphism (SSAP) system is the most popular and displayed a higher level of polymorphism than that of AFLPs in cucumber. In this study, we studied the genetic variation of the early generations of the synthetic allotetraploids Cucumis hytivus using DNA-SSAP marker systems in Cucumis. The results showed that allopolyploid formation was associated with rapid and extensive genomic changes. Most genomic changes involved loss of parental restriction fragments and appearance of novel fragments. In addition, the variation of the allotetraploids early generations was a rapid process, the loss of parental fragments and the gaining of novel fragments were mainly occurred in F1 hybrid, the S1 and S2 generations, and then stabilized. | |
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