ISHS Acta Horticulturae 820: VIII International Mango Symposium
USES OF ALTERNATIVE ANTIBIOTICS TO CONTROL BACTERIUM GROWTH IN MANGO EXPLANTS |
| Authors: | S. Rocha Monteiro de Andrade, F.G. Faleiro, J. Batista Teixeira, A.C. de Queiroz Pinto, G. Radel, M.C. Rocha Cordeiro, V.H. Vargas Ramo |
| Keywords: | plant tissue culture, decontamination, micropropagation, antibiotic |
| DOI: | 10.17660/ActaHortic.2009.820.69 |
| Abstract:
Bacterias, if they are not eliminated from the explant, compete for nutrients from the culture medium and also eliminate toxins in the medium modifying the normal development of the explant. The decontamination consists of microorganisms elimination, however preserving plant explant to be regenerated. The objective of this work was to develop a protocol to control an endogenous bacterium of mango internodal segments for the introduction in plant tissue culture medium. The explants had been submitted to treatments with ethanol, sodium hypoclorite and benomyl, for surface decontamination. The evaluation demonstrated that the bacterium is gram positive and resistant to the antibiotics rifampycin (300 mg.L-1), kanamycin (150 mg.L-1), ampicylin (150 mg.L-1), tetracyclin (150 mg.L-1), chloramphenicol (150 mg.L-1), carbenicyllin (150 mg.L-1) and streptomycin (300 mg.L-1). However, the results demonstrated that the combination of sulphametaxazole + trimetroprim (80 mg.L-1 + 16 mg.L-1) or sulfamethoxazole (80 mg.L-1) alone showed satisfactory result on the control of this endogenous bacterium, while trimethoprim (16 mg.L-1) did not work alone. Preliminary studies demonstrated that inclusion of copper sulphate (25 and 50 mg.L-1) into the nutritive medium controlled the endogenous fungi, however did not control of the bacterium growth. Medium containing 1% of propolis extract had also controlled the bacterium development, however the explants growth had also been affected. | |
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