ISHS Acta Horticulturae 789: XV Meeting of the EUCARPIA Tomato Working Group
TRANSFORMATION OF TOMATO WITH COLD-INDUCED SOLANUM SOGARANDINUM DHN24 DEHYDRIN GENE |
| Authors: | M. Głodek, G. Bartoszewski, Z. Yin, T. Rorat, K. Niemirowicz-Szczytt |
| Keywords: | cold stress, Lycopersicon esculentum, transgenic plant |
| DOI: | 10.17660/ActaHortic.2008.789.42 |
| Abstract:
Transgenic tomato plants expressing cold-induced 24 kDa dehydrin gene were obtained using an Agrobacterium-mediated transformation. Transgenic tomato shoots were regenerated from cotyledon explants inoculated with an Agrobacterium tumefaciens strain harboring binary plasmid carrying the Solanum sogarandinum 24 kDa dehydrin genes under a control of anthocyanidine glucosyltransferase promoter. Regenerated shoots were rooted and transferred to the greenhouse. All R0 plants showed normal morphology, seed set and were PCR positive. Kanamycin resistant R1 plants were selected and self-pollinated. Individual homozygous R1 plants were selected based on kanamycin resistance segregation of R2 families. Five transgenic lines carrying GT-dhn24 transgene have been developed and evaluated for the transgene expression in cold-stress conditions. GT-dhn24 transgene was expressed in all five transgenic lines. The transcript level was not increased by applied cold-treatment. | |
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