ISHS Acta Horticulturae 726: IV International Symposium on Pistachios and Almonds
IN VITRO CLONAL PROPAGATION OF PRUNUS PERSICA L. × PRUNUS AMYGDALUS BATSCH. HYBRID |
| Authors: | A. Imani, H. Abdollahi |
| Keywords: | micropropagation, meristem culture, rooting, sorbitol. |
| DOI: | 10.17660/ActaHortic.2006.726.27 |
| Abstract:
Some P. persica × P. amygdalus hybrids have shown adaptability to calcareous soils. In order to obtain appropriate rootstocks for almond and peach with more potential for rooting, the hybrid seeds between P. persica (genotype P-32) and P. amygdalus (genotype A-53) were germinated after 700 h exposure to 4°C. The hybrids were transferred to the in vitro condition and successfully established by meristem culture techniques, using 0.5 mm long apical domes. Test of different basic salts showed better growth and more survival of shootlets on WPM basic salts, but proliferation and length of shootlets were not affected. Increasing BAP concentrations from 1 to 2 mgl-1 caused more proliferation and long survival of hybrids but addition of 2ip (6-Dimethylallylaminopurine) did not. Using a combination of sucrose (2.5%) and sorbitol (0.5%) instead of sucrose alone (3.0%) improved proliferation of hybrids. Finally, root induction on the in vitro microcuttings was sufficiently induced by 4-5 weeks exposure to 1-2 mgL-1 IBA in the dark and subsequent transfer of microcuttings to a hormone free medium. | |
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