S-ALLELE GENOTYPE OF APPLE CULTIVARS AND SELECTIONS

The S-allele genotype of 25 apple cultivars and selections were determined by the polymerase chain reaction (PCR)-digestion identification method. ‘Adam’s Pearmain’ was assigned as S₁S₃, which differed from previous assignments as S₁₀S₁₁ by pollination tests (Kobel et al., 1939) and as S_10AS₁₁ by stylar protein analyses (Boskovic and Tobutt, 1999). Genome analyses and our pollination tests confirmed the S₁S₃ genotype. ‘Hida’, ‘Scarlet’, ‘Sekihikari’ and ‘Yukari’ were assigned as S₃S₉, S₃S₉, S₃S₃₀₍₂₈₎ and S2S9, respectively. The S-genotypes were not matched with any of the expected S-genotypes from supposed parents. Since the S-genotypes were in agreement with those determined by our pollination tests, the applicability of the S-genotype assignments based on the PCR-digestion identification method was confirmed.

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