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ISHS Acta Horticulturae 538: Eucarpia symposium on Fruit Breeding and Genetics

INFLUENCE OF SCAB INOCULUM CONCENTRATION IN AN APPLE BREEDING PROGRAM FOCUSED ON QUANTITATIVE RESISTANCE

Authors:   M. Lateur, B. Lefrancq, C. Wagemans
Keywords:   artificial inoculation
DOI:   10.17660/ActaHortic.2000.538.43
Abstract:
Since 1988, thousands of hybrids with selected old cultivars emerging from our genebank as parents are yearly produced at the department with the aim of selecting new commercial cultivars with low disease susceptibility. The objective is to create apple cvs possessing durable disease resistance to scab, powdery mildew and Nectria canker, combined with good fruit quality, high yield and long storage ability. One of the early steps of the selection process consists in testing the hybrid progenies for their resistance to scab. With the aim of standardising the inoculation scab test conditions, the seedlings are quantitatively inoculated in a bench of original conception by spraying conidia suspended in distilled water. This equipment guaranteeing a very homogeneous distribution of the liquid, therefore the influence of scab inoculum concentration can be studied. A range of 5 concentrations ( 1500, 15000, 37500, 150000 and 300000 conidia.ml-1) have been applied on the progenies of several parents in open pollination. Results show that in our experimental conditions only the weakest concentration (1500 conidia.ml-1) provides significant differences in the expression of the susceptibility to scab.

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