ISHS Acta Horticulturae 381: International Symposium on Natural Phenols in Plant Resistance
ELICITOR MEDIATED PHYTOALEXIN BIOSYNTHESIS IN TRANSGENIC ROOT CULTURES OF LOTUS CORNICULATUS. |
| Authors: | P. Morris, B. Thomas, M.P. Robbins |
| DOI: | 10.17660/ActaHortic.1994.381.24 |
| Abstract:
Agrobacterium rhizogenes transformed root cultures of Lotus corniculatus responded to both biotic and abiotic elicitors resulting in the de-novo synthesis of the typical Lotus isoflavan phytoalexins vestitol and sativan, which accumulated in both tissue and culture medium. Down regulation of constitutive tannin biosynthesis by auxin resulted in accumulation of chalcones and superinduction of phytoalexins on elicitation whereas down regulation of tannins with an antisense DFR gene did not result in chalcone accumulation or in modified phytoalexin accumulation. The elicitation of Lotus root cultures with GSH also caused changes in root tip morphology due to differential injury to root meristem cells. | |
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