ISHS Acta Horticulturae 381: International Symposium on Natural Phenols in Plant Resistance
AN ‘ANTISENSE’ STRATEGY FOR THE GENETIC MANIPULATION OF CONDENSED TANNIN AND ISOFLAVONOID PHYTOALEXIN ACCUMULATION IN TRANSGENIC LOTUS CORNICULATUS L. |
| Authors: | S.P. Colliver, M.P. Robbins, P. Morris |
| DOI: | 10.17660/ActaHortic.1994.381.12 |
| Abstract:
An antisense chalcone synthase gene construct made using the cDNA for chalcone synthase from Phaseolus vulgaris was introduced into the genome of three clonal genotypes of Lotus corniculatus via Agrobacterium rhizogenes. After initial screening 30 antisense (AS) and 15 control (C) transformation events were analysed and regenerative ability was found to be reduced in AS root cultures. AS compared to C root cultures appear to have a higher growth rate which may relate to ability to accumulate tannin. | |
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