ISHS Acta Horticulturae 380: Symposium on Tropical Root Crops in a Developping Economy
MANIPULATION OF CULTURAL FACTORS TO INCREASE MICROTUBER SIZE AND FREQUENCY IN SHOOT CULTURES OF FOOD YAM, DIOSCOREA ALATA L. CV. ORIENTAL LISBON |
| Authors: | A.Y. Alhassan, S.H. Mantell |
| DOI: | 10.17660/ActaHortic.1994.380.53 |
| Abstract:
The effects of supplementation of kinetin (KIN) with White's medium macrosalts, Heller's medium microsalts (T), and the Murashige-Skoog (MS) medium on microtuberization of Dioscorea alata cv. Oriental Lisbon shoot cultures were investigated. The results indicated that supplementing T medium with 1.0 μM KIN substantially improved microtuber frequencies. Interaction of KIN at all levels (1.0, 2.5, and 5.0 μM) with a set level of 1.0 μM abscisic acid (ABA) reduced microtuber frequency (by 14%) and increased individual microtuber weights (by 6%). The T medium without ABA and MS salts yielded similar levels of microtuber frequency. Microtubers produced were large enough (ca 100 mg) for field planting. Some shoot parameters were strongly correlated (r > 0.70) with microtuber frequency and weights. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
