ISHS Acta Horticulturae 319: International Symposium on Transplant Production Systems
EFFECTS OF 5,6-Cl2-IAA ON PROTOPLAST CULTURE AND CALLUS GROWTH IN RICE AND TOBACCO |
| Authors: | J.C. Chen, D. Zhang, L.F. Wang, Y.J. Zhao |
| DOI: | 10.17660/ActaHortic.1992.319.38 |
| Abstract:
The effects of 5,6-Cl2-IAA on senescence of tobacco callus were investigated. The results show that both the fresh weight and dry weight of callus cultured on MS medium supplemented with 0.02 or 0.2 mg/l 5,6-Cl2-IAA, were apparently higher than those of callus incubated on MSo medium (control) or MS medium supplemented with 2 mg/l 2,4-D. And it was observed that the growth of calli cultured on medium without 5,6-Cl2-IAA for 22 days was accompanied by tissue browning. In contrast, the callus cultured on medium with 5,6-Cl2-IAA could maintain normal growth. This role of 5,6-Cl2-IAA in delaying callus senescence may be related to the promoting effects of 5,6-Cl2-IAA on the activity of SOD isoenzymes. In addition, studies about the effects of 5,6-Cl2-IAA and other some plant hormones on rice protoplast culture indicate that 5,6-Cl2-IAA, 5,6-Cl2-IAA+KT or KT can induce formation of callus from rice protoplasts, but IAA can not induce the formation of callus. 5,6-Cl2-IAA also benefits further differentiation of callus from rice protoplasts. Regeneration plants were obtained from protoplasts of rice. | |
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