ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
TOWARDS GENETIC TRANSFORMATION OF BEAN BY AGROBACTERIUM TUMEFACIENS. |
| Authors: | A. Genga, A. Allavena, A. Ceriotti, R. Bollini |
| DOI: | 10.17660/ActaHortic.1990.280.87 |
| Abstract:
The relative ability of different bean genotypes to produce tumours following inoculation with several wild-type strains of Agrobacterium was determined both in vivo and in vitro. The cultivars tested were susceptible to most of the bacterial strains, but a genotype specific response for both the frequency and the size of induced tumours was observed. Engineered strains carrying a kanamycin resistance determinant were used for leaf disk cocultivation experiments. Callus tissues, which developed from leaf disk cut edges, were able to grow for several subcultures onto antibiotic-containing medium. The transformed nature of these calli was confirmed by assaying neomycin phosphotransferase (NPTII) activity. | |
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