ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
REGENERATION FROM ALSTROEMERIA CALLUS |
| Authors: | E. Gonzalez Benito, P.G. Alderson |
| DOI: | 10.17660/ActaHortic.1990.280.21 |
| Abstract:
Callus was obtained from mature embryos of a diploid variety of Alstroemeria ('Butterfly') on MS basal medium supplemented with either 2 or 4 mgl-1 picloram or 4 mgl-1 2,4-D combined with several concentrations (0 to 4 mgl-1) of BA or kinetin. Cultures were incubated at 25°C in darkness. After three weeks the compact yellowish callus produced was transferred to MS medium supplemented with 0.2 gl-1 case in hydrolysate (enzymatic) and 2 or 4 mgl-1 of BA or kinetin, and incubated at 25°C under a 16/8 hr photoperiod. Four weeks later, small white torpedo-like structures were observed on the surface of calli grown on 2 mgl-1 picloram + 2 mgl-1 BA, 4 mgl-1 picloram + 0.02 mgl-1 BA and 4 mgl-1 2,4-D + 2 mgl-1 BA. Subsequently shoots developed from these structures. On the surface of leaves of some regenerated shoots, similar structures were observed (secondary regeneration). Histological studies showed that both shoot regeneration and somatic embryogenesis had occurred. | |
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