Bitki Büyüme Düzenleyicilerinin Crepis bithynica (Asteraceae)’nın Sürgün Çoğaltımı Üzerine Etkileri: Türkiye’de Tehlike Altında Nadir Bir Tür
Abstract
Çalışmanın amacı: Bu çalışmada, bitki büyüme düzenleyicilerinin (PGRs) Türkiye’de tehlike altında nadir bir tür olan Crepis bithynica Boiss. (Asteraceae)’nın sürgün çoğaltımı üzerine etkileri araştırılmıştır.
Çalışma alanı: Bitki örnekleri, Kastamonu Ilgaz Dağı Milli Parkı’ndan toplanmıştır.
Materyal ve yöntem: Nodal segmentler eksplant olarak kullanıldı ve bunlar farklı bitki büyüme düzenleyicileri ile desteklenmiş Murashige and Skoog (MS) temel besiyerlerinde kültüre edildi. Çoğaltılan bitkilerdeki morfolojik değişmeler 21 gün boyunca in vitro kültürlerde izlendi.
Temel sonuçlar: Tüm bitki büyüme düzenleyicileri kallus oluşturmaksızın sürgün büyüme ve gelişmesini teşvik etmişlerdir. Bununla birlikte, eksplant başına en yüksek sürgün sayısı ve sürgün uzunluğu, sırasıyla 4.4 µM 6-BA ile 0.5 µM NAA ve 4.9 µM 2iP ile 0.5 µM IBA içeren MS besiyerlerinde elde edilmiştir.
Araştırma vurguları: Bu çalışma, C. bithynica’nın sürgün çoğaltımında PGR’lerin etkileri üzerine ilk çalışmadır ve elde edilen sonuçlar türün mikroçoğaltımı için kullanılabilir.
References
- Corral, P., Mallon, R., Rodriguez-Oubina, J. & Luz Gonzalez M. (2010). Multiple shoot induction and plant regeneration of the endangered species Crepis novoana. Plant Cell Tissue and Organ Culture (PCTOC), 105, 211-217.
- Inceer, H. & Aksu Kalmuk, N. (2019). Conservation assessment of some rare and endemic Crepis (Asteraceae) taxa in Turkey. Nature Conservation Research, 4(3), 117-123.
- Inceer, H., Cuce, M., Imamoglu, K.V., Ergin, T. & Ucler, A.O. (2022). In vitro propagation and cytogenetic stability of Tripleurospermum insularum (Asteraceae)–a critically endangered insular endemic species from Turkey. Plant Biosystems, 156(5), 1213-1221.
- Kim, K.H., Park, H.K., Park, M.S. & Yea, U.D. (2001). Effects of auxin and cytokinin on organogenesis of soybean Glycine max L. Journal of Plant Biotechnology, 3(2), 95-100.
- Lamond, J.M. (1975). Crepis L. In P. H. Davis (ed.), Flora of Turkey and the East Aegean Islands. v. 5. Edinburgh, Edinburgh University Press, 814-841.
- Mitrofanova, I.V., Ivanova, N.N. & Mitrofanova, O.V. (2020). Rare endemic plants of the mountainous Crimea Crepis purpurea (Willd.) M. Bieb. and Scrophularia exilis Popl. preservation under in vitro gene bank conditions. Bulletin of the State Nikitsky Botanical Gardens, 136, 14-23.
- Mitrofanova, O.V., Ivanova, N.N., Lesnikova-Sedoshenko, N.P., Brailko, V.A., Zhdanova I.V., et al. (2021). Effect of light intensity on in vitro regeneration in some relict endemic species of the Crimean flora. Acta Horticulture, 1324, 27-34. ISHS 2021. DOI 10.17660/ActaHortic.2021.1324.4
- Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
- Nowakowska, K., Pacholczak, A. & Tepper, W. (2019). The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L. ‘Alba’. Rendiconti Lincei. Scienze Fisiche e Naturali, 30, 197-205.
- Silvia, J.A.T., Nezami-Alanagh, E., Barreal, M.E., Kher, M.M., Wicaksono, A., et al. (2020). Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions. Planta, 252(47), 1-35. https://doi.org/10.1007/s00425-020-03449-4
- Su, Y.H., Liu, Y.B. & Zhang, X.S. (2011). Auxin-cytokinin interaction regulates meristem Development. Molecular Plant, 4(4), 616-625.
- Sujatha, M. & Reddy, T.P. (1998). Differential cytokinin effectson the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L.). Plant Cell Reports, 17, 561-566.
- Trejgell, A., Michalska, M. & Tretyn, A. (2010). Micropropagation of Senecio macrophyllus M. Bieb.. Acta Biologica Cracoviensia Series Botanica, 52(1), 67-72.
- Yurukova-Grancharova, P. & Dimitrova, D. (2006). Cytoembryological study of Crepis bithynica (Asteraceae) from Bulgaria. Flora Mediterranea, 16, 33-43.
Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye
Abstract
Aim of study: In this study, the effects of plant growth regulators (PGRs) on shoot multiplication of Crepis bithynica Boiss. (Asteraceae), an endangered rare species in Türkiye were investigated.
Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park.
Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog (MS) basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures.
Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively.
Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.
References
- Corral, P., Mallon, R., Rodriguez-Oubina, J. & Luz Gonzalez M. (2010). Multiple shoot induction and plant regeneration of the endangered species Crepis novoana. Plant Cell Tissue and Organ Culture (PCTOC), 105, 211-217.
- Inceer, H. & Aksu Kalmuk, N. (2019). Conservation assessment of some rare and endemic Crepis (Asteraceae) taxa in Turkey. Nature Conservation Research, 4(3), 117-123.
- Inceer, H., Cuce, M., Imamoglu, K.V., Ergin, T. & Ucler, A.O. (2022). In vitro propagation and cytogenetic stability of Tripleurospermum insularum (Asteraceae)–a critically endangered insular endemic species from Turkey. Plant Biosystems, 156(5), 1213-1221.
- Kim, K.H., Park, H.K., Park, M.S. & Yea, U.D. (2001). Effects of auxin and cytokinin on organogenesis of soybean Glycine max L. Journal of Plant Biotechnology, 3(2), 95-100.
- Lamond, J.M. (1975). Crepis L. In P. H. Davis (ed.), Flora of Turkey and the East Aegean Islands. v. 5. Edinburgh, Edinburgh University Press, 814-841.
- Mitrofanova, I.V., Ivanova, N.N. & Mitrofanova, O.V. (2020). Rare endemic plants of the mountainous Crimea Crepis purpurea (Willd.) M. Bieb. and Scrophularia exilis Popl. preservation under in vitro gene bank conditions. Bulletin of the State Nikitsky Botanical Gardens, 136, 14-23.
- Mitrofanova, O.V., Ivanova, N.N., Lesnikova-Sedoshenko, N.P., Brailko, V.A., Zhdanova I.V., et al. (2021). Effect of light intensity on in vitro regeneration in some relict endemic species of the Crimean flora. Acta Horticulture, 1324, 27-34. ISHS 2021. DOI 10.17660/ActaHortic.2021.1324.4
- Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
- Nowakowska, K., Pacholczak, A. & Tepper, W. (2019). The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L. ‘Alba’. Rendiconti Lincei. Scienze Fisiche e Naturali, 30, 197-205.
- Silvia, J.A.T., Nezami-Alanagh, E., Barreal, M.E., Kher, M.M., Wicaksono, A., et al. (2020). Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions. Planta, 252(47), 1-35. https://doi.org/10.1007/s00425-020-03449-4
- Su, Y.H., Liu, Y.B. & Zhang, X.S. (2011). Auxin-cytokinin interaction regulates meristem Development. Molecular Plant, 4(4), 616-625.
- Sujatha, M. & Reddy, T.P. (1998). Differential cytokinin effectson the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L.). Plant Cell Reports, 17, 561-566.
- Trejgell, A., Michalska, M. & Tretyn, A. (2010). Micropropagation of Senecio macrophyllus M. Bieb.. Acta Biologica Cracoviensia Series Botanica, 52(1), 67-72.
- Yurukova-Grancharova, P. & Dimitrova, D. (2006). Cytoembryological study of Crepis bithynica (Asteraceae) from Bulgaria. Flora Mediterranea, 16, 33-43.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | March 24, 2023 |
| Published in Issue | Year 2023 Volume: 23 Issue: 1 |
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