Vet Med - Czech, 2023, 68(5):208-217 | DOI: 10.17221/105/2022-VETMED

Beta-lactam, aminoglycoside, and quinolone resistance in Escherichia coli strains isolated from shrimps and mussels in the Marmara SeaOriginal Paper

B Celik ORCID...1, B Ergul ORCID...2, AI Kekec ORCID...1, B Halac ORCID...1, B Maslak ORCID...3, B Diren Sigirci ORCID...1, B Basaran Kahraman ORCID...1, AF Bagcigil ORCID...1, K Metiner ORCID...1, S Ak1
1 Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University – Cerrahpasa, Buyukcekmece/Istanbul, Turkiye
2 Ambarli Veterinary Border Control Point Directorate, Republic of Turkiye Ministry of Agriculture and Forestry, Istanbul, Turkiye
3 Institute of Graduate Studies, Istanbul University – Cerrahpasa, Avcilar/Istanbul, Turkiye

The purpose of the study was to examine the prevalence of Escherichia coli in shrimps and mussels, and to determine the distribution of β-lactam, aminoglycoside, quinolone, and multi-drug resistance phenotypically and genotypically in E. coli isolates obtained from mussels and shrimps in Istanbul. Faecal samples were collected from mussels (n = 96) and shrimps (n = 96) from the Marmara Sea coastline and fish markets in Istanbul. For the detection of antibiotic susceptibilities, seven antibiotic groups were used. β-lactamase, aminoglycoside, and quinolone genes were also determined. A total of 34 (17.7%, 15 shrimps, and 19 mussels) E. coli were isolated, and 17 (50%) were found to be resistant to one or more antimicrobials. The highest resistance was seen against aminoglycosides with 11 isolates (32.35%), followed by quinolones with 10 isolates (29.41%) and extended-spectrum β-lactamase (ESBL) with 4 isolates (11.76%). Multi-drug resistance was detected in 5 isolates (14.7%) from 3 shrimp and 2 mussel samples. The prevalence of ESBL genes was demonstrated at 3.84% in mussels and shrimp samples. There were no AmpC and carbapenemase-producing genes. These samples harbored blaCTX-M-1 (n = 3) and blaTEM (n = 4). Ten isolates were resistant to aminoglycosides genotypically. Resistance genes detected were strB in 2 isolates, aadA in 5, strB and aadA together in 3, ANT('')-Ia, aphA1 and aphA2 simultaneously in 3, aphA1 in 1, aac(3)-IIa in 1 isolate. aac(6')-Ib-cr gene was detected in only one of 10 phenotypically resistant isolates to quinolones.

Keywords: aquatic animals; E. coli; ESBL; multiple drug resistance

Received: December 6, 2022; Revised: April 27, 2023; Accepted: May 10, 2023; Published: May 29, 2023  Show citation

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Celik B, Ergul B, Kekec A, Halac B, Maslak B, Diren Sigirci B, et al.. Beta-lactam, aminoglycoside, and quinolone resistance in Escherichia coli strains isolated from shrimps and mussels in the Marmara Sea. Vet Med-Czech. 2023;68(5):208-217. doi: 10.17221/105/2022-VETMED.
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