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Class 1 and class 2 integrons and plasmid-mediated antibiotic resistance in coliforms isolated from ten rivers in northern Turkey

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Abstract

We aimed to determine the molecular mechanisms of antibiotic resistance in coliforms isolated from ten rivers in northern region of Turkey. A total of 183 isolates were tested for antimicrobial susceptibility by disk diffusion and agar dilution methods. Resistance to ampicillin, streptomycin, trimethoprim, tetracycline, and chloramphenicol was detected in 58%, 51.9%, 24%, 28.4%, and 12.5%, respectively. Twelve (6.5%) phylogenetically distant organisms were detected to harbor self-transmissible plasmids ranging 52 to >147 kb in sizes. Resistances to ampicillin, tetracycline, trimethoprim, streptomycin, and nalidixic acid were commonly transferable traits. Transferable nalidixic acid-resistant strains harbored qnrS gene, which was the first report of plasmid-mediated quinolone resistance in bacteria of environmental origin in Turkey. Fourteen and five coliforms harbored class 1 and class 2 integrons, respectively, and some of them were located on transferable plasmids. Sequence analyses of variable regions of the class 1 and 2 integrons harbored various gene cassettes, dfrA1, dfr2d, dfrA7, dfrA16, dfrA17, aadA1, aadA5, bla oxA-30, and sat1. A gene cassette array, dfrA16 has been demonstrated for the first time in a Citrobacter koseri isolate. Class 1 and class 2-bearing strains were clustered in different groups by BOX-PCR fingerprinting. Rivers in the northern Turkey may act as receptacle for the multi-drug resistant enterobacteria and can serve as reservoirs of the antimicrobial resistance determinants in the environment. The actual risk to public health is the transfer of resistance genes from the environmental bacteria to human pathogens.

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Authors and Affiliations

  1. Microbiology and Molecular Biology Research Laboratory, Department of Biology, Faculty of Arts and Sciences, Rize University, 53100, Rize, Turkey

    Osman Birol Ozgumus, Cemal Sandalli, Ali Sevim & Elif Celik-Sevim

  2. Department of Environmental Engineering, Faculty of Engineering, Istanbul University, 34320, Avcilar, Istanbul, Turkey

    Nuket Sivri

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  1. Osman Birol Ozgumus
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  2. Cemal Sandalli
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  3. Ali Sevim
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  4. Elif Celik-Sevim
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  5. Nuket Sivri
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Correspondence to Osman Birol Ozgumus.

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This study was presented in part at the 2nd World Conference on Magic Bullets, held October 3–5, 2008 in Nurnberg, Germany.

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Ozgumus, O.B., Sandalli, C., Sevim, A. et al. Class 1 and class 2 integrons and plasmid-mediated antibiotic resistance in coliforms isolated from ten rivers in northern Turkey. J Microbiol. 47, 19–27 (2009). https://doi.org/10.1007/s12275-008-0206-z

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  • DOI: https://doi.org/10.1007/s12275-008-0206-z

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