Koutsouveli, Vasiliki; Cárdenas, Paco; Rapp, Hans Tore; Riesgo, Ana (2020): Transcriptomic and histological analysis (Light and Transmission Electron Microscopy) on five Geodia species to identify their reproductive status and the gene expression during gametogenesis [dataset]. Natural History Museum, PANGAEA, https://doi.org/10.1594/PANGAEA.911273
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Abstract:
The collected samples of Geodia species were used to identify if they were reproductive and understand which genes are expressed during gametogenesis. Specifically, specimens fixed in glutaraldehyde solution, were processed for histological analysis to observe gametes in the sponge tissue. Once, we identified male, female (while in gametogenesis) and non-reproductive specimens, we used the RNAlater fixed samples to extract RNA from those specimens. Then, we prepared cDNA libraries, sequenced them with illumina nextseq and we did differential gene expression analysis in order to compare the expressed genes in male vs female specimens and vs non-reproductive specimens. The goal was to understand the molecular machinery of gametogenesis in sponges from an evolutionary point of view.
Supplement to:
Koutsouveli, Vasiliki; Cárdenas, Paco; Santodomingo, Nadia; Marina, Anabel; Morato, Esperanza; Rapp, Hans Tore; Riesgo, Ana (2020): The Molecular Machinery of Gametogenesis in Geodia Demosponges (Porifera): Evolutionary Origins of a Conserved Toolkit across Animals. Molecular Biology and Evolution, https://doi.org/10.1093/molbev/msaa183
Project(s):
Funding:
Horizon 2020 (H2020), grant/award no. 679849: Deep-sea Sponge Grounds Ecosystems of the North Atlantic
Coverage:
Median Latitude: 67.382866 * Median Longitude: 8.832116 * South-bound Latitude: 58.876230 * West-bound Longitude: 5.373950 * North-bound Latitude: 73.982650 * East-bound Longitude: 21.375000
Date/Time Start: 2016-04-05T00:00:00 * Date/Time End: 2017-07-29T00:00:00
Minimum Elevation: -2959.0 m * Maximum Elevation: -267.0 m
Event(s):
GS2016109A-06-ROV-01 * Latitude Start: 73.815650 * Longitude Start: 7.519170 * Latitude End: 73.982650 * Longitude End: 7.523700 * Date/Time Start: 2016-06-20T15:34:00 * Date/Time End: 2016-06-21T00:10:00 * Elevation Start: -717.0 m * Elevation End: -640.0 m * Location: Schultz Bank * Campaign: GS2016109A (GS16A-202) * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: 4 transects on summit (approx 900 m long and 100-120 m between lines)
GS2017110-09-ROV-6 * Latitude Start: 64.074900 * Longitude Start: 8.027000 * Latitude End: 64.077100 * Longitude End: 8.024000 * Date/Time Start: 2017-07-23T07:40:00 * Date/Time End: 2017-07-23T12:07:00 * Elevation Start: -293.0 m * Elevation End: -267.0 m * Location: Sula reef * Campaign: GS2017110 * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: From base and across the reef. Trawl bag and lines at 10.22.
GS2017110-23-ROV12 * Latitude Start: 73.790500 * Longitude Start: 6.853160 * Latitude End: 73.770480 * Longitude End: 6.973800 * Date/Time Start: 2017-07-28T23:16:00 * Date/Time End: 2017-07-29T07:39:00 * Elevation: -2959.0 m * Location: Schultz Bank * Campaign: GS2017110 * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: Northern slope of Schultz. Start on sedimentary plain at approx 3000 m
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Koutsouveli, Vasiliki | |||
| 2 | Latitude of event | Latitude | Koutsouveli, Vasiliki | |||
| 3 | Longitude of event | Longitude | Koutsouveli, Vasiliki | |||
| 4 | Sample code/label | Sample label | Koutsouveli, Vasiliki | |||
| 5 | Species | Species | Koutsouveli, Vasiliki | |||
| 6 | Investigator | Investigator | Koutsouveli, Vasiliki | person identifying species | ||
| 7 | Sex | Sex | Koutsouveli, Vasiliki | or non-reproductive status | ||
| 8 | DATE/TIME | Date/Time | Koutsouveli, Vasiliki | Geocode | ||
| 9 | Device type | Device | Koutsouveli, Vasiliki | |||
| 10 | Area/locality | Area | Koutsouveli, Vasiliki | |||
| 11 | Depth, bathymetric | Bathy depth | m | Koutsouveli, Vasiliki | water depth or range min | |
| 12 | Depth, bathymetric | Bathy depth | m | Koutsouveli, Vasiliki | range max |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
245 data points