[Purpose] The purpose of this study was to investigate electromyographic activity of the quadriceps muscle in different positions of hip rotation (while standing on the toes) in healthy females. [Participants and Methods] The study was conducted on 35 healthy females. Electromyographic activity of the rectus femoris, vastus medialis oblique, and vastus lateralis muscles were recorded with the hip in a neutral position as well as different angles of internal and external rotation (15, 30, and 45 degrees (°)) while the participants were standing on their toes. [Results] There was a significant difference of 15° in external rotation, a neutral position, 15° of internal rotation, and at the end of an internal rotation position. Paired comparison between muscle activity using Bonferroni showed that vastus lateralis activity in 15° of external rotation, a neutral position, 15° of internal rotation, and at the end of a position of internal rotation was more than that of rectus femoris muscle. [Conclusion] The present study showed that average vastus lateralis activity was the highest in all positions. However, the positions studied in the current study did not lead to an increase in muscle activity of the vastus medialis oblique as a medial dynamic stabilizer compared to vastus lateralis.