The effect of vincristine on morpho-functional state of neurons of cerebrospinal sensitive ganglions in experiment
DOI:
https://doi.org/10.15587/2519-8025.2017.93979Keywords:
vincristine, neurotoxicity, cerebrospinal ganglion, neurons, gliocytes, neurotubes, neurofilaments, stage, disorderAbstract
Vincristine is a preparation of vegetable generation, gotten from the vegetable rose myrtle (Vinca rosea L.), belongs to the group of chemotherapeutic preparations with expressed neurotoxicity. The active use of vincristine in the treatment of malignant tumors, especially in child age, conditions the study of influence of this preparation on peripheral nervous system. In this connection we studied morpho-functional state of neurons and gliocytes of rat cerebrospinal ganglion under effect of vincristine in experiment.
Aim of the work – to study the morpho-functional state of neurons and gliocytes of sensitive cerebrospinal rat ganglion under effect of vincristine in experiment.
Task of research – to reveal the disorder of structural components of sensitive cerebrospinal ganglion, conditioned by vincristine use.
Methods of research. The complex of light optical, morphometric and electromicroscopic methods of research was used to reveal the effect of vincristine use on structural components of sensitive cerebrospinal ganglion. The experimental study was carried out on 31 rats of both sexes (16 – experimental and 15 control ones) with mass 200,0–220, 0 g. The animals were kept in vivarium with free access to the food and water according to bioethics requirements as to the treatment of experimental animals, testified by correspondent act of bioethical expert opinion.
Results of research. The features of vincristine use in oncology for treating patients with malignant tumors of different organs and systems of organism and also the side effects of this preparation were considered. On the base of gotten electromicroscopic data we established the vincristine effect on the nervous structures of sensitive cerebrospinal ganglion in pathogenesis of vincristine-induced peripheral neuropathy. Thus, during the experiment there was observed the expressed sensitivity of neurons of sensitive cerebrospinal ganglions to the toxic effect of preparation. The big neurons suffer most, the small ones – least.
Conclusions. It was established, that vincristine effect is manifested by the disturbance of neurotubes and neurofilaments in esodic neurons with lesion of protein-synthesizing organobodies
References
- Bulkina, Z. P. (1978). Protivoopuholevyie preparatyi. Kyiv: Naukova dumka, 304.
- Blohin, N. N., Perevodchikova, N. I. (1984). Himioterapiya opuholevyih zabolevaniy. Moscow: Meditsina, 304.
- Swain, S. M., Arezzo, J. C. (2008). Neuropathy associated with microtubule inhibitors: diagnosis, incidence and management. Clin. Adv. Hematol. Oncol, 6 (6), 455–467.
- Huddart, R. A., Lau, F. N., Guerrero-Urbano, T., Jay, G., Norman, A., Horwich, A., Dearnaley, D. P. (2001). Accelerated Chemotherapy in the Treatment of Urothelial Cancer. Clinical Oncology, 13 (4), 279–283. doi: 10.1053/clon.2001.9269
- Dearnaley, D. P., Fossa, S. D., Kaye, S. B., Cullen, M. H., Harland, S. J., Sokal, M. P. J. et. al. (2005). Adjuvant bleomycin, vincristine and cisplatin (BOP) for high-risk stage I non-seminomatous germ cell tumours: a prospective trial (MRC TE17). British Journal of Cancer, 92 (12), 2107–2113. doi: 10.1038/sj.bjc.6602624
- Balayssac, D., Cayre, A., Ling, B., Maublant, J., Penault-Llorca, F., Eschalier, A. et. al. (2008). Vincristine-Induced Neuropathy in the Rat Is Not Modified by Drug-Drug Interactions with the P-Glycoprotein Inhibitor Verapamil. Chemotherapy, 54 (5), 336–342. doi: 10.1159/000151540
- Nishikawa, M., Nagatomi, H., Chang, B.-J., Sato, E., Inoue, M. (2001). Targeting Superoxide Dismutase to Renal Proximal Tubule Cells Inhibits Mitochondrial Injury and Renal Dysfunction Induced by Cisplatin. Archives of Biochemistry and Biophysics, 387 (1), 78–84. doi: 10.1006/abbi.2000.2237
- Aley, K. O., Reichling, D. B., Levine, J. D. (1996). Vincristine hyperalgesia in the rat: A model of painful vincristine neuropathy in humans. Neuroscience, 73 (1), 259–265. doi: 10.1016/0306-4522(96)00020-6
- Topp, K. S., Tanner, K. D., Levine, J. D. (2000). Damage to the cytoskeleton of large diameter sensory neurons and myelinated axons in vincristine-induced painful peripheral neuropathy in the rat. The Journal of Comparative Neurology, 424 (4), 563–576. doi: 10.1002/1096-9861(20000904)424:4<563::aid-cne1>3.3.co;2-l
- Silva, A., Wang, Q., Wang, M., Ravula, S. K., Glass, J. D. (2006). Evidence for direct axonal toxicity in vincristine neuropathy. Journal of the Peripheral Nervous System, 11 (3), 211–216. doi: 10.1111/j.1529-8027.2006.0090.x
- Carozzi, V. A., Canta, A., Chiorazzi, A. (2015). Chemotherapy-induced peripheral neuropathy: What do we know about mechanisms? Neuroscience Letters, 596, 90–107. doi: 10.1016/j.neulet.2014.10.014
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