β-Naphthoflavone Caused Up-Regulation of AhR Regulated GFP in Transgenic Zebrafish

Seung-Hyeok SEOK; DukWoong PARK; Jong-Hwan PARK; Sun-A CHO; Min-Won BAEK; Hui-Young LEE; Dong-Jae KIM; BoHwan JIN; Doug-Young RYU; Jae-Hak PARK

doi:10.1538/expanim.53.479

Note

β-Naphthoflavone Caused Up-Regulation of AhR Regulated GFP in Transgenic Zebrafish

Seung-Hyeok SEOK, DukWoong PARK, Jong-Hwan PARK, Sun-A CHO, Min-Won BAEK, Hui-Young LEE, Dong-Jae KIM, BoHwan JIN, Doug-Young RYU, Jae-Hak PARK

Author information

Seung-Hyeok SEOK
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
DukWoong PARK
Environmental Health, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Jong-Hwan PARK
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Sun-A CHO
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Min-Won BAEK
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Hui-Young LEE
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Dong-Jae KIM
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
BoHwan JIN
Environmental Health, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Doug-Young RYU
Environmental Health, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Jae-Hak PARK
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

Keywords: aryl hydrocarbon receptor, green fluorescent protein, zebrafish

JOURNAL FREE ACCESS

2004 Volume 53 Issue 5 Pages 479-483

DOI https://doi.org/10.1538/expanim.53.479

Details

Abstract

A transgenic zebrafish strain was generated expressing the aryl hydrocarbon receptor (AhR)-regulated green fluorescent protein (GFP) reporter gene. Following exposure to β-naphthoflavone (βNF), the transgenic fish exhibited up-regulation of GFP in the face and vertebrae compared to vehicle controls. βNF-exposed fish exhibited gross dysmorphogenesis in vertebral development by 5 days after fertilization.

Corresponding author

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