1998 Volume 47 Issue 2 Pages 137-140
In our laboratory animal facility, Sendai virus (HVJ) contamination occurred in a negative flow rack used for experimental infection with 4 strains of mouse-adapted influenza virus (Inf.V). Anti-HVJ antibody (Ab) was detected in 35/42 mice in the rack. To specify the strain of Inf.V contaminated with HVJ, experimental infection was performed by using A, B and D strains of Inf.V in each vinyl isolator. Anti-HVJ Ab was detected in all mice infected with A strain at day 28 post-infection. As a result of experimental infection with A strain of Inf.V which was treated with anti-HVJ mouse serum, the virus suspension was determined not to contain HVJ and allowed for experimental use in our facility. Since then, HVJ contamination has not occurred in our facility.
Bulletin of the Experimental Animals
Proceedings of The Japanese Association of Animal Models for Human Diseases
Proceedings of The Japanese Society of Animal Models for Human Diseases
