Abstract
Objectives
In this study, we determined the gastroprotective and ulcer-healing effects of extracts (aqueous and methanolic) of Nauclea pobeguinii stem-back.
Methods
Gastroprotective and healing activity were evaluated following a HCl/ethanol and an indomethacin-induced acute ulcers models; acetic acid, pylorus-ligature, pylorus ligature/histamine and pylorus ligature/acetylcholine-induced chronic ulcers models.
Results
It emerges from this study that, at 100, 200 and 400 mg/kg, the extracts significantly reduced the various ulceration parameters. Compared to negative control male rats, the aqueous (100 mg/kg) and methanolic (400 mg/kg) extracts of Nauclea pobeguinii inhibited the ulcers induced by HCl/ethanol by 80.76 % and 100 % respectively, as well as ulcers induced by indomethacin by 88.28 % and 93.47 % respectively. Animals that received 200 mg/kg of both extracts showed a significant reduction in the levels of monocytes, lymphocytes, nitric oxide, MDA and a significant increase in the activities of SOD and catalase. Histological analysis showed repaired mucous epithelium at all doses of both extracts. Aqueous and methanol extracts inhibited ulceration indices by 89.33 % and 88.53 % for pylorus ligature, 83.81 % and 61.07 % for pylorus ligature/acetylcholine and 87.29 % and 99.63 % for pylorus ligature/histamine respectively. Both extracts protected the stomach lining with percentages inhibition of 79.49 % and 81.73 %, respectively in the ethanol test. The extracts induced a significant increase in mucus mass (p<0.001).
Conclusions
The aqueous and methanol extracts of Nauclea pobeguinii healed ulcers thanks to their anti-inflammatory, anti-oxidant, anti-secretory and cytoprotective properties.
-
Research funding: None declared.
-
Author contributions: MM, AG and MKYK designed the work. MKYK, AG, MM, FZNL, NAE, TEG, DNSF, ACF and MMVM collected and analysed the data. MM, MKYK and AG drafted, read and revised critically the manuscript. All authors accept to be accountable for all aspects of the work.
-
Competing interests: The authors declare no conflicts of interest regarding this article.
-
Informed consent: Not applicable.
-
Ethical approval: All the procedures described in this document (use of animals for experimentation) were in absolute respect for ethics, the preservation, breeding and handing of the fauna and flora of Cameroon (Ethics Committee of the Cameroonian Ministry of Scientific Research and Technology) adopted the guidelines (European Union on animal care and testing) (Council CCE 86/609).
-
Data availability: All data supporting our findings are adequately contained within the manuscript.
References
1. Konturek, PC, Konturek, JW, Konturek, SJ. Gastric secretion and the pathogenesis of peptic ulcer in the Helicobacter pylori infection. J Physiol Pharmacol 1996;47:5–19.Search in Google Scholar
2. Heyam, M, Sidahmed, Najihah, S, Siddig, I, Manal, M, Taha, et al.. Evidence of the gastroprotective and anti-Helicobacter pylori activities of β-mangostin isolated from Cratoxylum arborescens (vahl) blume. Dove Press Journal 2016;10:297–313. https://doi.org/10.2147/DDDT.S80625.Search in Google Scholar
3. Ateufack, G, Domgnim Mokam, EC, Mbiantcha, M, Dongmo Feudjio, RB, Nana, D, Kamanyi, A. Gastroprotective and ulcer healing e ffects of Piptadeniastrum Africanum on experimentally induced gastric ulcers in rats. BMC Compl Alternative Med 2015;15:214. https://doi.org/10.1186/s12906-015-0713-5.Search in Google Scholar
4. Matah Marthe, VM, Ateufack, G, Mbiantcha, M, Yousseu Nana, W, Atsamo, AD, Adjouzem, FC, et al.. Cytoprotective and antisecretory properties of methanolic extract of Distemonanthus benthamianus (Caesalpiniaceae) stem bark on acute gastric ulcer in rats. J Complement Integr Med 2020;18:37–49. https://doi.org/10.1515/jcim-2019-0216.Search in Google Scholar
5. N’Guessan, K, Kadja, B, Ziritti, GN, Traoré, D, Ake-Assi, L. Screening phytochimique de quelques plantes médicinales ivoiriennes utilisées en pays Krobou. Sci Nat 2009;6:1–15.Search in Google Scholar
6. Bali, A. Drug treatment of peptic ulcer disease. England: Peptic Ulcer Disease; 2016:1–19 pp.Search in Google Scholar
7. Machowska, A, Szlachcic, A, Pawlik, M, Brzozowski, T, Konturek, SJ, Pawlik, WW. The role of female and male sex hormones in the healing process of preexisting lingual and gastric ulcerations. J Physiol Pharmacol 2004;55:91–104.Search in Google Scholar
8. Aston, NO, Kalaichandra, S, Carr, JV. Duodenal ulcer hemorrhage in the puerperium. Can J Surg 1991;34:482–3.Search in Google Scholar
9. Mbiantcha, M, Tsafack, EG, Ateufack, G, Nana, YW, Bomba, T, Djuichou, NSF, et al.. Analgesic, anti-inflammatory and anti-arthritic properties of aqueous and methanolic stem bark extracts from Nauclea pobeguinii (Rubiaceae) in rats. J Compl Integr Med 2018;15:1–7.Search in Google Scholar
10. Messia, K, Tona, L, Mapunza, L, Ntamabyaliro, J, Muanda, T, Muyembe, T, et al.. Antimalarial efficacy of a quantified extract of Nauclea pobeguinii sterm bPraark on human volunteer. Planta Med 2012;7:234–48.Search in Google Scholar
11. Seukep, J, Sandjo, L, Ngadjui, B, Kuate, V. Antibacterial activity of the extract and compounds from Nauclea pobeguinii against Gram negative multi drug. BMC Compl Alternative Med 2016;16:181–93.10.1186/s12906-016-1173-2Search in Google Scholar
12. Kadiri, H, Adegor, E, Asagba, S. Effects of aqueous Nauclea pobeguinii leaf extracts on rat induced with hepatic injury. Res J Med Plant 2007;1:139–43.10.3923/rjmp.2007.139.143Search in Google Scholar
13. Mfotie, N, Munvera, A, Mkounga, P, Ngenfack, A, Gaw, J. Phytochemical analysis with free radical scavenging nitric oxide, inhibition and antiproliferative activity of Sarcocephalus pobeguinii extract. Complement Altern Med 2017;17:199.10.1186/s12906-017-1712-5Search in Google Scholar
14. Messia, K, Tona, G, Peuge, O, Lusaki, B, Nanga, T, Cimanga, R, et al.. Anti-malaria activities and toxicities of three plants used as traditional remedies for malaria in the Democratic Republic of Congo: Croton mubango, Nauclea pobeguinii and Pyrenacantha standii. Ann Trop Med Parasitol 2005;99:345–57. https://doi.org/10.1179/136485905X36325.Search in Google Scholar
15. Sofidia, O, Lilian, A, Abidemi, J, Johson, A, Oluwole, B. Effect of Flabellia paniculata cav. extracts on gastric ulcer in rats. Complement Altern Med 2012;12:168–74.10.1186/1472-6882-12-168Search in Google Scholar
16. Khan, A, Hussain, S, Jais, A, Zakaria, Z, Khan, M. Antiulcer activity of ficus religiosa stem bark ethanolic extract in rats. J Med Plants Res 2011;5:354–9.Search in Google Scholar
17 Shahrokhi, N, Keshavarzi, Z, Khaksari, M. Ulcer healing activity of Mumijo aqueous extract against acetic acid induced gastric ulcer in rats. J Pharm Bioallied Sci 2015;7:56–59.10.4103/0975-7406.148739Search in Google Scholar
18 Maheswari, B, Rajyalakshmi, DP, Ajith, K, VedPrakash, P, SeshaSai Gayatri, K. Evaluation of antiulcer activity of ethanol extract leaves of Lactuca Sativa. J drug deliv ther 2020;10:196–199.10.22270/jddt.v10i4.4190Search in Google Scholar
19. Amang, A, Paul, V, Nkwengoua, E, Nyasse, B. Antisecretory action of the extract of the aerial parts of eremomastax speciosa occurs through antihistaminic and anticholinergic pathways. Hindawi Publishing Corporation. Adv Pharmacol Sci 2014;2014:1–10.10.1155/2014/323470Search in Google Scholar PubMed PubMed Central
20. Nyam, K, Tang, J, Long, K. Anti-ulcer activity of Hibiscus cannabinus and Hibiscus sabdariffa seeds in ulcer-induced rats. Int Food Res J 2016;23:1164–72.Search in Google Scholar
21. Lamarque, D. Physiopathologie des lésions gastroduodénales induites par les anti-inflammatoires non stéroïdiens. Gastroentérol Clin Biol 2004;28:18–26. https://doi.org/10.1016/s0399-8320(04)95275-x.Search in Google Scholar PubMed
22. Tsafack, EG, Djuichou Nguemnang, SF, Atsamo, AD, Nana Yousseu, W, Tadjoua, TH, Matah, MMV, et al.. In vitro anti-inflammatory, anti-oxidant and in vivo antiarthritic properties of stem bark extracts from Nauclea pobeguinii (Rubiaceae) in rats. Asian Pac J Trop Biomed 2020;10:65–77.10.4103/2221-1691.275421Search in Google Scholar
23. Felipe, M, Ana, C, Anderson, L, Ricardo, J, Christiane, T, Maria, S, et al.. Mechanism of action underlying the gastric ulcer activity of Rhizophora mangle L. J Ethnopharmacol 2011;139:234–43, https://doi.org/10.1016/j.jep.2011.11.007.Search in Google Scholar PubMed
24. N’Guessan, K, Kadja, B, Ziritti, GN, Traoré, D, Ake-Assi, L. Screening phytochimique de quelques plantes médicinales ivoiriennes utilisées en pays Krobou. Sci Nat 2009;6:1–15.10.4314/scinat.v6i1.48575Search in Google Scholar
25. Sameer, U, Mrutyunjay, M, Ramabhimaiah, S. Evaluation of the antiulcerogenic effect of Zingiber officinal (Ginger) root in rats. Int j curr microbiol 2014;1:347–54.Search in Google Scholar
26. Hiruma-Lima, C, Calvo, T, Rodriguez, C, Andrade, F. Antiulcerogenic activity of Archonea castaneafolio effects on stomatostatin, gastrin and prostaglandin. J Ethnopharmacol 2006;104:215–21. https://doi.org/10.1016/j.jep.2005.09.007.Search in Google Scholar PubMed
27. Sabiha, S, Mohd, AA, Asif, M, Akhtar, M. Role of phenolic compounds in peptic ulcer: an overview. J Pharm BioAllied Sci 2011;3:361–367. https://doi.org/10.4103/0975-7406.84437.Search in Google Scholar PubMed PubMed Central
28. Kelly, SLM, Guilherme, END, Meri, EFP, Ânderson, LF, Alba, RMSB, Clélia, AHL, et al.. Flavonoids with gastroprotective activity. Molecules 2009;14:979–1012. https://doi.org/10.3390/molecules14030979.Search in Google Scholar PubMed PubMed Central
29. Almeida, AL, Beleza, MLML, Campos, A, Rosa, RL, Andrade, SF, Filho, VC, et al.. Phytochemical profile and gastroprotective potential of Myrcianthes pungens fruits and leaves. Nutrire 2017;42:24. https://doi.org/10.1186/s41110-017-0040-3.Search in Google Scholar
30. Santos, LD, Campos, A, Cechinel Filho, V, Nesello, LAN. Phytochemical profile and gastroprotective activity of Eugenia mattosii fruits. Arq Gastroenterol 2018. https://doi.org/10.1590/S0004-2803.201800000-24.Search in Google Scholar PubMed
31. Périco, LL, Rodrigues, PV, Ohara, R, Bueno, G, Nunes, VV, Santos, RC, et al.. Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats. World J Gastroenterol 2018;24:4369–83. https://doi.org/10.3748/wjg.v24.i38.4369.Search in Google Scholar PubMed PubMed Central
32. Wenji, Z, Yingyi, L, Qiuhua, L, Lingli, S, Ruohong, C, Xingfei, L, et al.. Preventive ant therapeutic potential of flavonoids in gastric ulcers. Molecules 2020;25:1–31.Search in Google Scholar
33. Rashedunnabi, AM, In-Shik, K, Dongchoon, A, Hyun-Jin, T, Hyeon-Hwa, N, Byung-Kil, C, et al.. Anti-inflammatory and gastroprotective roles of rabdosia inflexa through downregulation of pro-inflammatory cytokines and MAPK/NF-κB signaling pathways. Int J Mol Sci 2018;19:584. https://doi.org/10.3390/ijms19020584.Search in Google Scholar PubMed PubMed Central
34. Adediran, O, Avwioro, O, Funsho-Agun, A. A study of ulcer induced stomach of albino rats treated with idogu mixture, a potent anti-ulcer herbal drug. Afr J Cell Path 2017;8:21–6. https://doi.org/10.5897/ajcpath17.004.Search in Google Scholar
35. Prazeres, LD, Ticiana, PA, Brito, SA, Almeida, CF, Silva, AD, de paula, MM, et al.. Antioxidant and antiulcerogenic activity of the dry extract of pods of Libidibia ferrea Mart. ex. Tul. (Fabaceae). Oxid Med Cell Longev 2019;2019:1–23. https://doi.org/10.1155/2019/1983137.Search in Google Scholar PubMed PubMed Central
36. Sahoo, N, Gu, M, Zhang, X, Samuelson, L, Merchant, J, Xu, H, et al.. Gastric acid secretion from parietal cells is mediated by a ca2+ efflux channel in the tubulovesicle. Dev Cell 2017;41:262–73. https://doi.org/10.1016/j.devcel.2017.04.003.Search in Google Scholar PubMed PubMed Central
37. Engevik, AC, Kaji, I, Goldenring, JR. The physiology of the gastric parietal cell. Physiol Rev 2020;100:573–602. https://doi.org/10.1152/physrev.00016.2019.Search in Google Scholar PubMed PubMed Central
38. Aihara, T, Nakamura, E, Amagase, K, Tomita, K, Fujishita, T, Furutani, K, et al.. Pharmacological control of gastric acid secretion for the treatment of acid-related peptic disease: past, present and future. Pharmacol Ther 2013;98:109–27. https://doi.org/10.1016/s0163-7258(03)00015-9.Search in Google Scholar PubMed
39. Ekokon, J, Umoh, F, Udobang, J, Etim, I. Antiulcerogenic activity of ethanolic leaf extract of. Croton zambesicus Muell Arg Afr J Biomed Res 2011;14:43–7.Search in Google Scholar
© 2023 Walter de Gruyter GmbH, Berlin/Boston
