Abstract
Background
Vitiligo, a skin disorder is viewed as a multifactorial process with major role of reactive oxygen species in concert to destroy or incapacitate melanocytes. In Unani system of medicine the treatment of Bars (Vitiligo) starts with removal of harmful materials from the body with Munzij and Mushil (MM), a poly herbal Unani formulation.
Methods
Herein, oxidative stress related parameters as MDA, SOD, GPx and CAT have been estimated in the 21 clinically diagnosed Vitiligo in-patients and subsequently these parameters were evaluated during and after administration of MM therapy and compared with 21 healthy subjects.
Results
There was significant difference in the parameters viz., SOD (p<0.001) and CAT (p<0.005) activity at the baseline with no statistical significant difference in MDA and GPx activity among Vitiligo subjects and controls. After MM therapy there was no statistical significant difference among the values of these parameters in Vitiligo subjects.
Conclusions
The results suggest that there is imbalance in the oxidant-antioxidant status of Vitiligo subjects and the MM therapy is not found to significantly change the levels of oxidative stress related parameters.
Acknowledgments
We would like to acknowledge Hon'ble DG CCRUM for infrastructure support. We hereby appreciate the participation of the patients as well as the cooperation of the staff especially of Pharmacy section of CRIUM Hyderabad. Further we would like to acknowledge Mrs Nasreen and Ms Jabeen for their help during experimentation.
Author contributions: None declared.
Research funding: Central Council for Research in Unani Medicine, Ministry of AYUSH, Govt. of India and Department of Science & Technology, Ministry of Science & Technology, Govt. of India.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: None declared.
References
[1] Sehgal VN, Srivastava G. Vitiligo: compendium of clinico-epidemilogical features. Indian J Dermatol Venereol Leprol 2007;73:149–56.10.4103/0378-6323.32708Search in Google Scholar
[2] Yildirim M, Baysal V, Inaloz HS, Kesici D, Delibas N. The role of oxidants and antioxidants in generalized vitiligo. J Dermatol 2003;30:104–8.10.1111/j.1346-8138.2003.tb00356.xSearch in Google Scholar
[3] Monograph on bars (vitiligo) published by CCRUM. Department of AYUSH, Ministry of Health & Family Welfare, Govt. of India 2006:11–14.Search in Google Scholar
[4] Clinical studies on bars (vitiligo) published by CCRUM. Ministry of Health & Family Welfare, Govt. of India 1986;18:70–3.Search in Google Scholar
[5] Nikam PS, Nikam SV, Sontakke AV. Lipid peroxidation and antioxidants during treatment of hepatitits-b with phyllanthus amarus. Int J Biol Med Res 2010;1:41–3.Search in Google Scholar
[6] Prasad T, Kundu MS. Serum IgG and IgM responses to sheep red blood cells (SRBC) in weaned calves fed milk supplemented with Zn and Cu. Nutrition 1995;11:712–15.Search in Google Scholar
[7] Hazneci E, Karabulut AB, Ozturk C, Batcioglu K, Dogan G, Karaca S, et al. A comparative study of superoxide dismutase, catalase, and glutathione peroxidase activities and nitrate levels in vitiligo patients. Int J Dermatol 2005;44:636–40.10.1111/j.1365-4632.2004.02027.xSearch in Google Scholar
[8] Pugliese PT. The skin’s antioxidant systems. Dermatol Nurs 1998;10:401–16.Search in Google Scholar
[9] Shajil EM, Begum R. Antioxidant status of segmental and non-segmental vitiligo. Pigment Cell Res 2006;19:179–80.10.1111/j.1600-0749.2006.00299.xSearch in Google Scholar
[10] Agrawal D, Shajil EM, Marfatia YS, Begum R. Study on the antioxidant status of vitiligo patients of different age groups in Baroda. Pigment Cell Res 2004;17:289–94.10.1111/j.1600-0749.2004.00149.xSearch in Google Scholar
[11] Arican O, Kurutas EB. Oxidative stress in the blood of patients with active localized Vitiligo. Acta Dermatovenerol APA 2008;17:12–16.Search in Google Scholar
[12] Thomas CE, Morehouse LA, Aust SD. Ferritin and superoxide-dependent lipid peroxidation. J Biol Chem 1985;260:3275–80.10.1016/S0021-9258(19)83617-7Search in Google Scholar
[13] Dammak I, Boudaya S, Ben Abdallah F, Turki H, Attia H, Hentati B. Antioxidant enzymes and lipid peroxidation at the tissue level in patients with stable and active vitiligo. Int J Dermatol 2009;48:476–80.10.1111/j.1365-4632.2009.03998.xSearch in Google Scholar PubMed
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