Abstract
To obtain rapeseed protein with low phytic acid (PA), soy protein isolate (SPI) was used to investigate the interactions between SPI and PA. The influence of pretreatment (soaking using salt solution and dialysis) of the defatted rapeseed meal on the PA and protein content in the final rapeseed proteins was also studied. The results showed that electrostatic interactions dominated the protein–PA interaction, which was affected by pH and ionic strength. Accordingly, the pH and ionic strength in the soaking medium also influenced the PA remained in the rapeseed proteins. The PA content decreased with the ionic strength (400–800 mM) and relatively low PA was obtained at pH 6.0 (soaking environment). Finally, 52.8 % of the PA have been removed and PA content remained in rapeseed protein isolate (RPI) reached about 0.84 mg/g, at the same time, the protein content was maintained around 86.70 %. Overall, soaking using salt solution and dialysis could be an effective method to achieve high quality rapeseed protein with low PA.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 32202048
Funding source: Scientific Research Foundation of the Hubei University of Technology
Award Identifier / Grant number: XJ2021001501
Funding source: Collaborative Grant-in-aid of the HBUT National “111” Center for Cellular Regulation and Molecular Pharmaceutics
Award Identifier / Grant number: XBTK-2021008
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: Unassigned
Funding source: Hubei University
Award Identifier / Grant number: Unassigned
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Research ethics: The local Institutional Review Board deemed the study exempt from review, and this study complies with local legal requirements.
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Author contributions: All the authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors declare no conflict of interest regarding the publication of this manuscript.
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Research funding: This study was financed by the National Natural Science Foundation of China (No. 32202048), the Scientific Research Foundation of the Hubei University of Technology (No. XJ2021001501) and the HBUT National “111” Center for Cellular Regulation and Molecular Pharmaceutics (XBTK-2021008).
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Data availability: Data will be made available upon request.
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