Abstract
The formation and the growth of regenerated fronds from cultivated barbs of Asparagopsis armata were investigated according to an experimental design methodology. A maximal ratio of 3.3 mm regeneration/mm barb was reached for two media constituted of seawater complemented with iron sulfate, EDTA and potassium bromide. The first optimal medium was composed of [FeSO47H2O] = 0.20 mg L−1, [Na2EDTA2H2O] = 2.9 mg L−1, [KBr] = 12.0 mg L−1 and the second one of [FeSO47H2O] = 0.2 mg L−1, [Na2EDTA2H2O] = 4.6 mg L−1, [KBr] = 28.7 mg L−1. Barbs excised from adult thalli were placed for 4 weeks in seawater enriched with several chemical substances at different concentrations. The experimental procedure involved: 1) the screening of the influencing compounds with an Hadamard design and 2) the investigation of their optimal concentrations according to a Doehlert design of response surface methodology.
Copyright (c)2001 by Walter de Gruyter GmbH & Co. KG
