Three mulberry genotypes viz., MR₂, C₇₆₃ and Morus macroura were analyzed for the stomatal frequency and karyotypic attributes. MR₂ and C₇₆₃ were found to be diploids with somatic chromosome number of 2n=28, while variety Morus cathyana revealed tetraploid chromosome number of 2n=56. Somatic chromosomes measured 1.26 to 2.86 µm in length. Karyotypes of these taxa are symmetric. Only metacentric and sub-metacentric chromosomes are found in the complement. Stomatal frequency was found to be high in diploid mulberry varieties compared to tetraploid variety.