STUDIES ON THE CHOLESTATIC FACTOR : Partial Purification of the Cholestatic Factor
Keywords:
cholestatic factor
1986 Volume 35 Issue 4 Pages 290-294
Details
1986 Volume 35 Issue 4 Pages 290-294
The cholestatic factor showing potent intrahepatic activity was produced from activated lymphocytes of tuberculin-sensitized guinea pigs by stimulating them with PPD. Intrahepatic activity was observed in two fractions obtained by gel filtration on Sephadex G-75 followed by DEAE-cellulose column chromatography. When these two fractions were analyzed by isoelectric focusing electrophoresis, they were found to contain different electric charges: one with the isoelectric point at 3.0-3.5, the other at pH 7.8-8.0.
