薄層クロマトグラフフィー及び3α-ハイドロキシステロイドーデヒドロゲナー
ゼによる尿中プレグナントリオールの測定
1976 年 5 巻 1 号 p. 140-143
詳細
1976 年 5 巻 1 号 p. 140-143
Determination of urinary pregnanetriol using 3α-hydroxysteroid-dehydrogenase was described. It is based on the specific enzymatic convertion of 3α-hydroxy group on the steroid molecule into 3-keto group. Quantitation is obtained by measurment of the absorption at340nm from NADH, following a hydrolysis by β-glucuronidase, extraction with methylenechloride and purification by thin-layer chromatography. The main advantage of the method rests in its specificity of 3α-hydroxysteroid-dehydrogenase in comparison with the reaction of sulfuric acid.
