グルタチオンS-トランスフェラーゼを使用したグルタチオン分画定量法
1988 年 16 巻 3 号 p. 162-168
詳細
1988 年 16 巻 3 号 p. 162-168
A simple method for glutathione (GSH) and glutathione disulfide (GSSG) was devised, using glutathione reductase (GR) and glutathione S-transferase (GSTR).
The total concentration of glutathione (T-GSH) was determined by an enzymatic assay with the use of GR, and the concentration of GSSG was selectively determined after GSH had been eliminated by treating specimens with 1-chloro-2, 4-dinitrobenzene and GSTR.
Using the present method, the concentrations of GSH in normal human red blood cells ranged between 2.1-3.2μmol/1010 cells, whereas those of GSSG was lower than 0.1μmol/1010 cells.
